01763naa a2200253 a 450000100080000000500110000800800410001902400370006010000200009724500960011726000090021330000300022252009060025265000370115865000240119565000210121965000170124065000180125765000220127565000250129770000200132270000200134277301470136220931612018-09-18       2018    bl uuuu     u00u1 u    #d7 a0.1007/978-1-4939-8594-4_192DOI1 aSOUZA, F. V. D.  aCryopreservation of pollen grains of pineapple and other bromeliads.h[electronic resource]  c2018  a279-288cv.1815. Cap. 19.  aCryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform.  aBreeding and Genetic Improvement  aConservation plants  aCryopreservation  aBromeliaceae  aConservação  aCriopreservação  aMelhoramento Vegetal1 aSOUZA, E. H. de1 aSILVA, R. L. da  tIn: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018.