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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
31/10/2017 |
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Data da última atualização: |
13/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROSSI, G. B.; VALENTIM-NETO, P. A.; BLANK, M.; FARIA, J. C. de; ARISI, A. C. M. |
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Afiliação: |
GABRIELA BARBOSA ROSSI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; PEDRO ALEXANDRE VALENTIM-NETO, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MARTINA BLANK, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Comparison of grain proteome profiles of four brazilian common bean (Phaseolus vulgaris L.) cultivars. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, v. 65, n. 34, p. 7588-7597, Aug. 2017. |
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ISSN: |
0021-8561 |
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DOI: |
10.1021/acs.jafc.7b03220 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a source of proteins for about one billion people worldwide. In Brazil, ?BRS Sublime?, ?BRS Vereda?, ?BRS Esteio?, and ?BRS Estilo? cultivars were developed by Embrapa to offer high yield to farmers and excellent quality to final consumers. In this work, grain proteomes of these common bean cultivars were compared based on two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (MS/MS). Principal component analysis (PCA) was applied to compare 349 matched spots in these cultivars proteomes, and all cultivars were clearly separated in PCA plot. Thirty two differentially accumulated proteins were identified by MS. Storage proteins such as phaseolins, legumins, and lectins were the most abundant, and novel proteins were also identified. We have built a useful platform that could be used to analyze other Brazilian cultivars and genotypes of common beans. |
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Palavras-Chave: |
2-DE; PCA. |
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Thesagro: |
Feijão; Phaseolus vulgaris; Proteína. |
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Thesaurus Nal: |
Beans; Cultivars; Proteins; Proteome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01770naa a2200301 a 4500
001 2078517
005 2017-12-13
008 2017 bl uuuu u00u1 u #d
022 $a0021-8561
024 7 $a10.1021/acs.jafc.7b03220$2DOI
100 1 $aROSSI, G. B.
245 $aComparison of grain proteome profiles of four brazilian common bean (Phaseolus vulgaris L.) cultivars.$h[electronic resource]
260 $c2017
520 $aCommon bean (Phaseolus vulgaris L.) is a source of proteins for about one billion people worldwide. In Brazil, ?BRS Sublime?, ?BRS Vereda?, ?BRS Esteio?, and ?BRS Estilo? cultivars were developed by Embrapa to offer high yield to farmers and excellent quality to final consumers. In this work, grain proteomes of these common bean cultivars were compared based on two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (MS/MS). Principal component analysis (PCA) was applied to compare 349 matched spots in these cultivars proteomes, and all cultivars were clearly separated in PCA plot. Thirty two differentially accumulated proteins were identified by MS. Storage proteins such as phaseolins, legumins, and lectins were the most abundant, and novel proteins were also identified. We have built a useful platform that could be used to analyze other Brazilian cultivars and genotypes of common beans.
650 $aBeans
650 $aCultivars
650 $aProteins
650 $aProteome
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aProteína
653 $a2-DE
653 $aPCA
700 1 $aVALENTIM-NETO, P. A.
700 1 $aBLANK, M.
700 1 $aFARIA, J. C. de
700 1 $aARISI, A. C. M.
773 $tJournal of Agricultural and Food Chemistry$gv. 65, n. 34, p. 7588-7597, Aug. 2017.
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Embrapa Arroz e Feijão (CNPAF) |
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| 8. |  | QUEIROZ, E. F. de; MOLION, L. C. B.; MINOR, H. C.; NEUMAIER, N. Um modelo matemático de quantificação do efeito da disponibilidade hídrica em soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 31, n. 10, p. 683-690, out. 1996. Título em inglês: A mathematical model to quantify the effect of water availability on soybean.| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| Registros recuperados : 8 | |
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