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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
29/07/2015 |
Data da última atualização: |
18/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS-GONZALEZ, P. L.; SARUBBI-ORUE, H.; GONZALES-SEGNANA, L.; CHABI-JESUS, C.; ASTUA, J. de F.; KITAJIMA, E. W. |
Afiliação: |
PEDRO LUIS RAMOS-GONZALEZ, IAC; HUMBERTO SARUBBI-ORUE, Universidad Nacional de Asuncion; LUIS GONZALES-SEGNANA, Universidad Nacional de Asuncion; CAMILA CHABI-JESUS, IAC; JULIANA DE FREITAS ASTUA, CNPMF; ELIOT WATANABE KITAJIMA, ESALQ. |
Título: |
Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Journal of Phytopathology, 2015. |
Páginas: |
6p. |
Série: |
1439-0434 |
Idioma: |
Inglês |
Conteúdo: |
The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. |
Palavras-Chave: |
Citrus leprosis virus. |
Thesagro: |
Doença de planta; Orchidaceae; Orquídea. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 01522naa a2200253 a 4500 001 2020714 005 2023-05-18 008 2015 bl uuuu u00u1 u #d 100 1 $aRAMOS-GONZALEZ, P. L. 245 $aOrchid Fleck Virus Infecting Orchids in Paraguay$bFirst Report and Use of Degenerate Primers for its Detection.$h[electronic resource] 260 $c2015 300 $a6p. 490 $a1439-0434 520 $aThe presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. 650 $aDoença de planta 650 $aOrchidaceae 650 $aOrquídea 653 $aCitrus leprosis virus 700 1 $aSARUBBI-ORUE, H. 700 1 $aGONZALES-SEGNANA, L. 700 1 $aCHABI-JESUS, C. 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 773 $tJournal of Phytopathology, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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1. |  | MANFIO, C. E.; SATO, A. Y.; MOTOIKE, S. Y.; LOPES, F. de a.; LAVIOLA, B. G.; CONCEIÇÃO, L. D. H. C. S. DA. Diversidade genética entre progênies de macaúba na fase de muda. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 6., 2011, Búzios. Panorama atual e perspectivas do melhoramento de plantas no Brasil: anais. [Búzios]: SBMP, 2011. Não paginado.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Agroenergia; Embrapa Cerrados. |
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2. |  | MASSON, M. V.; TAVARES, W. de S.; LOPES, F. de A.; SOUZA, A. R. de; FERREIRA-FILHO, P. J.; BARBOSA, L. R.; WILCKEN, C. F.; ZANUNCIO, J. C. Selitrichodes neseri (Hymenoptera: Eulophidae) recovered from Leptocybe invasa (Hymenoptera: Eulophidae) galls after initial release on Eucalyptus (Myrtaceae) in Brazil, and data on its biology. Florida Entomologist, v. 100, n. 3, p. 589-593, Sept. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Florestas. |
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