01522naa a2200253 a 450000100080000000500110000800800410001910000260006024501390008626000090022530000080023449000140024252007910025665000220104765000160106965000140108565300260109970000210112570000250114670000200117170000200119170000200121177300370123120207142023-05-18       2015    bl uuuu     u00u1 u    #d1 aRAMOS-GONZALEZ, P. L.  aOrchid Fleck Virus Infecting Orchids in ParaguaybFirst Report and Use of Degenerate Primers for its Detection.h[electronic resource]  c2015  a6p.  a1439-0434  aThe presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples.  aDoença de planta  aOrchidaceae  aOrquídea  aCitrus leprosis virus1 aSARUBBI-ORUE, H.1 aGONZALES-SEGNANA, L.1 aCHABI-JESUS, C.1 aASTUA, J. de F.1 aKITAJIMA, E. W.  tJournal of Phytopathology, 2015.