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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/03/2015 |
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Data da última atualização: |
24/03/2015 |
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Autoria: |
HERINGER, A. S.; STEINMACHER, D. A.; FRAGA, H. P. F.; VIEIRA, L. N.; REE, J. F.; GUERRA, M. P. |
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Título: |
Global DNA methylation profiles of somatic embryos of peach palm (Bactris gasipaes Kunth) are influenced by cryoprotectants and droplet-vitrification cryopreservation. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Plant Cell Tissue and Organ Culture, v. 114, p. 365-372, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Regrowth capacity and genetic stability of plants recovered following cryopreservation are associated with changes in DNA epigenetics, particularly in DNA methylation levels. In this study, global DNA methylation profiles associated with frequency of regrowth of peach palm ( Bactris gasipaes ) somatic embryos following cryo- preservation using droplet-vitrification were investigated. Somatic embryo clusters (SEC) subjected to plant vitrifi- cation solution 3 (PVS3) for different durations (0, 60, 120, 180, and 240 min) were evaluated for regrowth capacity. The highest frequency of regrowth (52.4 %) was obtained when SEC were incubated in PVS3 for 120 min prior to droplet-vitrification cryopreservation. Global DNA meth- ylation profiles were influenced by both cryoprotectants and droplet-vitrification cryopreservation. Incubation of SEC in PVS3 for limited durations not only reduced fre- quency of regrowth, but also increased DNA methylations levels when compared with proliferating SEC grown in a temporary immersion system. Although SEC subjected to cryopreservation exhibited the highest DNA methylation variation, 120 min SEC incubation in a PVS3 solution resulted in the recovery of initial global methylation pro- files after 24 weeks of regrowth. |
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Palavras-Chave: |
Espécie florestal; Pupunheira. |
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Thesagro: |
Bactris Gasipaes; Criopreservação; Embriogénese; Espécie Nativa; Palmito; Pupunha. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02137naa a2200277 a 4500
001 2012013
005 2015-03-24
008 2013 bl uuuu u00u1 u #d
100 1 $aHERINGER, A. S.
245 $aGlobal DNA methylation profiles of somatic embryos of peach palm (Bactris gasipaes Kunth) are influenced by cryoprotectants and droplet-vitrification cryopreservation.$h[electronic resource]
260 $c2013
520 $aRegrowth capacity and genetic stability of plants recovered following cryopreservation are associated with changes in DNA epigenetics, particularly in DNA methylation levels. In this study, global DNA methylation profiles associated with frequency of regrowth of peach palm ( Bactris gasipaes ) somatic embryos following cryo- preservation using droplet-vitrification were investigated. Somatic embryo clusters (SEC) subjected to plant vitrifi- cation solution 3 (PVS3) for different durations (0, 60, 120, 180, and 240 min) were evaluated for regrowth capacity. The highest frequency of regrowth (52.4 %) was obtained when SEC were incubated in PVS3 for 120 min prior to droplet-vitrification cryopreservation. Global DNA meth- ylation profiles were influenced by both cryoprotectants and droplet-vitrification cryopreservation. Incubation of SEC in PVS3 for limited durations not only reduced fre- quency of regrowth, but also increased DNA methylations levels when compared with proliferating SEC grown in a temporary immersion system. Although SEC subjected to cryopreservation exhibited the highest DNA methylation variation, 120 min SEC incubation in a PVS3 solution resulted in the recovery of initial global methylation pro- files after 24 weeks of regrowth.
650 $aBactris Gasipaes
650 $aCriopreservação
650 $aEmbriogénese
650 $aEspécie Nativa
650 $aPalmito
650 $aPupunha
653 $aEspécie florestal
653 $aPupunheira
700 1 $aSTEINMACHER, D. A.
700 1 $aFRAGA, H. P. F.
700 1 $aVIEIRA, L. N.
700 1 $aREE, J. F.
700 1 $aGUERRA, M. P.
773 $tPlant Cell Tissue and Organ Culture$gv. 114, p. 365-372, 2013.
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Registro original: |
Embrapa Florestas (CNPF) |
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