02137naa a2200277 a 450000100080000000500110000800800410001910000200006024501950008026000090027552012710028465000210155565000220157665000180159865000200161665000120163665000120164865300230166065300150168370000230169870000200172170000180174170000150175970000180177477300670179220120132015-03-24       2013    bl uuuu     u00u1 u    #d1 aHERINGER, A. S.  aGlobal DNA methylation profiles of somatic embryos of peach palm (Bactris gasipaes Kunth) are influenced by cryoprotectants and droplet-vitrification cryopreservation.h[electronic resource]  c2013  aRegrowth capacity and genetic stability of plants recovered following cryopreservation are associated with changes in DNA epigenetics, particularly in DNA methylation levels. In this study, global DNA methylation profiles associated with frequency of regrowth of peach palm ( Bactris gasipaes ) somatic embryos following cryo- preservation using droplet-vitrification were investigated. Somatic embryo clusters (SEC) subjected to plant vitrifi- cation solution 3 (PVS3) for different durations (0, 60, 120, 180, and 240 min) were evaluated for regrowth capacity. The highest frequency of regrowth (52.4 %) was obtained when SEC were incubated in PVS3 for 120 min prior to droplet-vitrification cryopreservation. Global DNA meth- ylation profiles were influenced by both cryoprotectants and droplet-vitrification cryopreservation. Incubation of SEC in PVS3 for limited durations not only reduced fre- quency of regrowth, but also increased DNA methylations levels when compared with proliferating SEC grown in a temporary immersion system. Although SEC subjected to cryopreservation exhibited the highest DNA methylation variation, 120 min SEC incubation in a PVS3 solution resulted in the recovery of initial global methylation pro- files after 24 weeks of regrowth.  aBactris Gasipaes  aCriopreservação  aEmbriogénese  aEspécie Nativa  aPalmito  aPupunha  aEspécie florestal  aPupunheira1 aSTEINMACHER, D. A.1 aFRAGA, H. P. F.1 aVIEIRA, L. N.1 aREE, J. F.1 aGUERRA, M. P.  tPlant Cell Tissue and Organ Culturegv. 114, p. 365-372, 2013.