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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
02/12/2008 |
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Data da última atualização: |
02/12/2008 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
LOURENTE, E. R. P.; MERCANTE, F. M.; TOKURA, A. M.; MIOTTO, D.; COLAÇO, F. W.; VIANA, C. M. |
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Afiliação: |
Elaine Reis Pinheiro Lourente, Uniderp; Fábio Martins Mercante, Embrapa Agropecuária Oeste; Alessandra Mayumi Tokura; Daniel Miotto; Fernando Winter; Cleython Marcel Viana. |
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Título: |
Atributos químicos e microbiológicos avaliados em sistemas de cultivos agrícolas e florestais. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 2., 2008, Dourados. A construção participativa da agroecologia em Mato Grosso do Sul: anais. Dourados: Embrapa Agropecuária Oeste, 2008. |
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Descrição Física: |
1 CD-ROM. |
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ISBN: |
978-85-7540-019-7 |
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Idioma: |
Português |
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Notas: |
Organizado por: Alberto Feiden, Milton Parron Padovan, Adalgiza Inês Campolim, Aurélio Vinícius Borsato, Ivo de Sá Motta, João Batista Catto, Tércio Jacques Fehlauer. |
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Thesagro: |
Microbiologia do Solo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00938naa a2200217 a 4500
001 1239028
005 2008-12-02
008 2008 bl uuuu u00u1 u #d
020 $a978-85-7540-019-7
100 1 $aLOURENTE, E. R. P.
245 $aAtributos químicos e microbiológicos avaliados em sistemas de cultivos agrícolas e florestais.
260 $c2008
300 $c1 CD-ROM.
500 $aOrganizado por: Alberto Feiden, Milton Parron Padovan, Adalgiza Inês Campolim, Aurélio Vinícius Borsato, Ivo de Sá Motta, João Batista Catto, Tércio Jacques Fehlauer.
650 $aMicrobiologia do Solo
700 1 $aMERCANTE, F. M.
700 1 $aTOKURA, A. M.
700 1 $aMIOTTO, D.
700 1 $aCOLAÇO, F. W.
700 1 $aVIANA, C. M.
773 $tIn: SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 2., 2008, Dourados. A construção participativa da agroecologia em Mato Grosso do Sul: anais. Dourados: Embrapa Agropecuária Oeste, 2008.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/04/2021 |
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Data da última atualização: |
13/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOUSA, T. P. de; CHAIBUB, A. A.; CORTES, M. V. de C. B.; BATISTA, T. F. C.; BEZERRA, G. de A.; SILVA, G. B. da; FILIPPI, M. C. C. de. |
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Afiliação: |
THATYANE PEREIRA DE SOUSA, UFG; AMANDA ABDALLAH CHAIBUB, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; TELMA FATIMA COELHO BATISTA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; GUSTAVO DE ANDRADE BEZERRA, UFG; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Archives of Microbiology, 2021. |
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ISSN: |
0302-8933 |
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DOI: |
https://doi.org/10.1007/s00203-021-02307-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. MenosThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M... Mostrar Tudo |
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Palavras-Chave: |
Antagonism; Bioagent; Mycoparasitism. |
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Thesagro: |
Antagonismo; Arroz; Brusone; Controle Biológico; Marcador Molecular; Oryza Sativa; Trichoderma. |
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Thesaurus NAL: |
Biological control; Mycoparasites; Rice. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02690naa a2200373 a 4500
001 2131247
005 2021-04-13
008 2021 bl uuuu u00u1 u #d
022 $a0302-8933
024 7 $ahttps://doi.org/10.1007/s00203-021-02307-5$2DOI
100 1 $aSOUSA, T. P. de
245 $aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.$h[electronic resource]
260 $c2021
520 $aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.
650 $aBiological control
650 $aMycoparasites
650 $aRice
650 $aAntagonismo
650 $aArroz
650 $aBrusone
650 $aControle Biológico
650 $aMarcador Molecular
650 $aOryza Sativa
650 $aTrichoderma
653 $aAntagonism
653 $aBioagent
653 $aMycoparasitism
700 1 $aCHAIBUB, A. A.
700 1 $aCORTES, M. V. de C. B.
700 1 $aBATISTA, T. F. C.
700 1 $aBEZERRA, G. de A.
700 1 $aSILVA, G. B. da
700 1 $aFILIPPI, M. C. C. de
773 $tArchives of Microbiology, 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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