02690naa a2200373 a 450000100080000000500110000800800410001902200140006002400520007410000200012624501610014626000090030752016140031665000230193065000180195365000090197165000160198065000100199665000120200665000240201865000230204265000170206565000160208265300150209865300130211365300190212670000190214570000270216470000220219170000220221370000200223570000250225577300360228021312472021-04-13       2021    bl uuuu     u00u1 u    #d  a0302-89337 ahttps://doi.org/10.1007/s00203-021-02307-52DOI1 aSOUSA, T. P. de  aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.h[electronic resource]  c2021  aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.  aBiological control  aMycoparasites  aRice  aAntagonismo  aArroz  aBrusone  aControle Biológico  aMarcador Molecular  aOryza Sativa  aTrichoderma  aAntagonism  aBioagent  aMycoparasitism1 aCHAIBUB, A. A.1 aCORTES, M. V. de C. B.1 aBATISTA, T. F. C.1 aBEZERRA, G. de A.1 aSILVA, G. B. da1 aFILIPPI, M. C. C. de  tArchives of Microbiology, 2021.