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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
28/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. |
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Afiliação: |
TATIANE ALMEIDA DRUMMOND TETZNER, UNESP/JABOTICABAL; NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA-USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTNA RAMIRES FERREIRA, UNICAMP/CAMPINAS; CLARA SLADE OLIVEIRA, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011. |
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DOI: |
https://doi.org/10.1590/S1516-35982011001000010 |
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Idioma: |
Inglês |
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Conteúdo: |
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. MenosEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryo; Protein source. |
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Thesaurus Nal: |
in vitro culture; ovalbumin. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49368/1/PROCI2011.00188.pdf
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Marc: |
LEADER 02467naa a2200253 a 4500
001 1908631
005 2022-07-28
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1516-35982011001000010$2DOI
100 1 $aTETZNER, T. A. D.
245 $aThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos.$h[electronic resource]
260 $c2011
520 $aEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
650 $ain vitro culture
650 $aovalbumin
653 $aBovine embryo
653 $aProtein source
700 1 $aSARAIVA, N. Z.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aFERREIRA, C. R.
700 1 $aOLIVEIRA, C. S.
700 1 $aGARCIA, J. M.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 10, p. 2135-2141, oct. 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
09/09/2022 |
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Data da última atualização: |
12/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JOVINO, R. S.; SILVA, T. R. da S.; RODRIGUES, R. T.; CARVALHO, J. R. de S.; CUNHA, J. B. de A.; LIMA, L. M. de; SANTOS, R. C. dos; SANTOS, C. E. de R. e S.; RIBEIRO, P. R. de A.; FREITAS, A. D. S. de; MARTINS, L. M. V.; FERNANDES-JÚNIOR, P. I. |
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Afiliação: |
RAPHAEL SILVA JOVINO, UEPB; THAÍSE ROSA DA SILVA, UNIVASF; RUTH TEREZINHA RODRIGUES, UNIVASF; JACKSON RAFAEL DE SÁ CARVALHO, UEFS; JUSSARA BARBOZA DE ALENCAR CUNHA, UNEB; LIZIANE MARIA DE LIMA, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA; CAROLINA ETIENNE DE ROSÁLIA E SILVA SANTOS, UFRPE; PAULA ROSE DE ALMEIDA RIBEIRO, FACEPE/CNPQ/CPATSA; ANA DOLORES SANTIAGO DE FREITAS, UFRPE; LINDETE MÍRIA VIEIRA MARTINS, UNEB; PAULO IVAN FERNANDES JÚNIOR, CPATSA. |
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Título: |
Elite Bradyrhizobium strains boost biological nitrogen fxation and peanut yield in tropical drylands. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 53, p. 1623-1632, jul. 2022. |
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ISSN: |
1678-4405 |
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DOI: |
https://doi.org/10.1007/s42770-022-00792-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efcient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efcient peanut rhizobia in experiments under diferent edaphoclimatic conditions. This work evaluated the agronomic efciency and the biological nitrogen fxation (BNF) by two elite Bradyrhizobium strains under four diferent felds in the Brazilian semiarid region. We compared a new efcient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil. Besides the inoculated treatments, two uninoculated controls were assessed (with and without 80 kg ha−1 of N-urea). The BNF was estimated by the δ15N approach in three out of four feld assays. BNF contribution was improved by inoculation of both Bradyrhizobium strains, ranging from 42 to 51% in Petrolina and 43 to 60% in Nossa Senhora da Glória. Peanuts? yields benefted from the inoculation of both strains and N fertilization in all four assays. Nevertheless, the results showed the efciency of both strains under diferent edaphoclimatic conditions, indicating the native strain ESA 123 as a potential bacterium for recommendation as inoculants for peanuts in Brazil, mainly in drylands. |
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Thesagro: |
Amendoim; Inoculante; Nitrogênio; Seleção; Semente; Solo Árido. |
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Thesaurus NAL: |
Arachis hypogaea subsp. hypogaea; Rhizobiaceae; Rhizobiales; Seed inoculation; Semiarid soils; Strains. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02722naa a2200421 a 4500
001 2146324
005 2022-09-12
008 2022 bl uuuu u00u1 u #d
022 $a1678-4405
024 7 $ahttps://doi.org/10.1007/s42770-022-00792-4$2DOI
100 1 $aJOVINO, R. S.
245 $aElite Bradyrhizobium strains boost biological nitrogen fxation and peanut yield in tropical drylands.$h[electronic resource]
260 $c2022
520 $aPeanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efcient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efcient peanut rhizobia in experiments under diferent edaphoclimatic conditions. This work evaluated the agronomic efciency and the biological nitrogen fxation (BNF) by two elite Bradyrhizobium strains under four diferent felds in the Brazilian semiarid region. We compared a new efcient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil. Besides the inoculated treatments, two uninoculated controls were assessed (with and without 80 kg ha−1 of N-urea). The BNF was estimated by the δ15N approach in three out of four feld assays. BNF contribution was improved by inoculation of both Bradyrhizobium strains, ranging from 42 to 51% in Petrolina and 43 to 60% in Nossa Senhora da Glória. Peanuts? yields benefted from the inoculation of both strains and N fertilization in all four assays. Nevertheless, the results showed the efciency of both strains under diferent edaphoclimatic conditions, indicating the native strain ESA 123 as a potential bacterium for recommendation as inoculants for peanuts in Brazil, mainly in drylands.
650 $aArachis hypogaea subsp. hypogaea
650 $aRhizobiaceae
650 $aRhizobiales
650 $aSeed inoculation
650 $aSemiarid soils
650 $aStrains
650 $aAmendoim
650 $aInoculante
650 $aNitrogênio
650 $aSeleção
650 $aSemente
650 $aSolo Árido
700 1 $aSILVA, T. R. da S.
700 1 $aRODRIGUES, R. T.
700 1 $aCARVALHO, J. R. de S.
700 1 $aCUNHA, J. B. de A.
700 1 $aLIMA, L. M. de
700 1 $aSANTOS, R. C. dos
700 1 $aSANTOS, C. E. de R. e S.
700 1 $aRIBEIRO, P. R. de A.
700 1 $aFREITAS, A. D. S. de
700 1 $aMARTINS, L. M. V.
700 1 $aFERNANDES-JÚNIOR, P. I.
773 $tBrazilian Journal of Microbiology$gv. 53, p. 1623-1632, jul. 2022.
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Embrapa Algodão (CNPA) |
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