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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Unidades Centrais. |
Data corrente: |
10/05/2010 |
Data da última atualização: |
10/05/2010 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
SILVA, R. J.; STEPHAN, M. P.; TEIXEIRA, K. R. dos S. |
Afiliação: |
RENATA JORGE DA SILVA, UFRRJ; MARILIA PENTEADO STEPHAN, CTAA; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB. |
Título: |
Interferência na quantificação de proteínas em cultura de Gluconacetobacter diazotrophicus em meio semi-sólido contendo glicose. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Seropédica: Embrapa Agrobiologia, 2009. |
Páginas: |
4 p. |
Série: |
(Embrapa Agrobiologia. Comunicado técnico, 121). |
Idioma: |
Português |
Conteúdo: |
Apresenta as observações sobre os efeitos interferentes da glicose e apresenta subsídios para uma abordagem alternativa para dosagem de proteínas quando glicose estiver presente no meio de cultivo em condições específicas. |
Palavras-Chave: |
Bactéria diazotrófica; Método de Lowry. |
Thesagro: |
Espectrofotometria; Glicose; Proteína. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 00918nam a2200217 a 4500 001 1783234 005 2010-05-10 008 2009 bl uuuu u0uu1 u #d 100 1 $aSILVA, R. J. 245 $aInterferência na quantificação de proteínas em cultura de Gluconacetobacter diazotrophicus em meio semi-sólido contendo glicose. 260 $aSeropédica: Embrapa Agrobiologia$c2009 300 $a4 p. 490 $a(Embrapa Agrobiologia. Comunicado técnico, 121). 520 $aApresenta as observações sobre os efeitos interferentes da glicose e apresenta subsídios para uma abordagem alternativa para dosagem de proteínas quando glicose estiver presente no meio de cultivo em condições específicas. 650 $aEspectrofotometria 650 $aGlicose 650 $aProteína 653 $aBactéria diazotrófica 653 $aMétodo de Lowry 700 1 $aSTEPHAN, M. P. 700 1 $aTEIXEIRA, K. R. dos S.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
24/06/2005 |
Data da última atualização: |
29/11/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - B |
Autoria: |
ALVES, R. M.; ARTERO, A. S.; SEBBENN, A. M.; FIGUEIRA, A. |
Afiliação: |
RAFAEL MOYSES ALVES, CPATU; ANGELA S. ARTERO, USP/CENA; ALEXANDRE M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO; ANTONIO FIGUEIRA, USP/CENA. |
Título: |
Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Genetics and Molecular Biology, v. 26, n. 3, p. 373-379, 2003. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate ($tm= 1.0) and individual outcrossing rate ($t= 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate ($ts) was elevated (0.946), but lower than $tm, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate ($tm-$ts). The estimation of genetic divergence ($FST) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity ($rp= 0.930) and the mean coefficient of co-ancestrality within families ($?xy= 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species breeding programs (half-sibs). MenosThe aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate ($tm= 1.0) and individual outcrossing rate ($t= 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate ($ts) was elevated (0.946), but lower than $tm, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate ($tm-$ts). The estimation of genetic divergence ($FST) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity ($rp= 0.930) and the mean coefficient of co-ancestrality within families ($?xy= 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species bree... Mostrar Tudo |
Palavras-Chave: |
Brasil; Conservação de germoplasma; Nova Ipixuna; Pará; População natural. |
Thesagro: |
Cupuaçu; Genética. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/18122/1/a25v26n3.pdf
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Marc: |
LEADER 02250naa a2200253 a 4500 001 1406930 005 2016-11-29 008 2003 bl uuuu u00u1 u #d 100 1 $aALVES, R. M. 245 $aMating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers. 260 $c2003 520 $aThe aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate ($tm= 1.0) and individual outcrossing rate ($t= 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied population the estimate for single locus outcrossing rate ($ts) was elevated (0.946), but lower than $tm, confirming the likely outcrossing character of the species and suggesting the occurrence of 5.4% biparental inbreeding rate ($tm-$ts). The estimation of genetic divergence ($FST) between allelic frequencies in ovules and pollen revealed a deviation from random mating in 75% of the evaluated loci. Likewise, the estimate of correlation of paternity ($rp= 0.930) and the mean coefficient of co-ancestrality within families ($?xy= 0.501) indicated that the outcrossings were predominantly correlated, and the offspring were full-sibs. These results suggested that for this particular population of T. grandiflorum, the sampling strategy for genetic conservation and breeding should adopt specific models for families derived from correlated outcrossing (full-sibs) and not the ones usually adopted in classic outcrossing species breeding programs (half-sibs). 650 $aAmazonia 650 $aCupuaçu 650 $aGenética 653 $aBrasil 653 $aConservação de germoplasma 653 $aNova Ipixuna 653 $aPará 653 $aPopulação natural 700 1 $aARTERO, A. S. 700 1 $aSEBBENN, A. M. 700 1 $aFIGUEIRA, A. 773 $tGenetics and Molecular Biology$gv. 26, n. 3, p. 373-379, 2003.
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Embrapa Amazônia Oriental (CPATU) |
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