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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
11/01/2019 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATTOS, B. B.; MONTEBIANCO, C.; ROMANEL, E.; SILVA, T. da F.; BERNABÉ, R. B.; SIMAS-TOSIN, F.; SOUZA, L. M.; SASSAKI, G. L.; VASLIN, M. F. S.; BARRETO-BERGTER, E. |
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Afiliação: |
BIANCA BRAZ MATTOS, CNPS; CAROLINE MONTEBIANCO, UFRJ; ELISSON ROMANEL, UFRJ/USP; TATIANE DA FRANCA SILVA, UFRJ/USP; RENATO BARROSO BERNABÉ, UFRJ; FERNANDA SIMAS-TOSIN, UFPR; LAURO M. SOUZA, UFPR; GUILHERME L. SASSAKI, UFPR; MAITE F. S. VASLIN, UFRJ; ELIANA BARRETO-BERGTER, UFRJ. |
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Título: |
A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 126, p. 206-216, May 2018. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2018.02.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively. MenosCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), a... Mostrar Tudo |
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Palavras-Chave: |
Peptidogalactomannan. |
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Thesagro: |
Defesa Vegetal; Glicoproteína. |
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Thesaurus Nal: |
Cladosporium herbarum. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 03116naa a2200289 a 4500
001 2103593
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2018.02.023$2DOI
100 1 $aMATTOS, B. B.
245 $aA peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells.$h[electronic resource]
260 $c2018
520 $aCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively.
650 $aCladosporium herbarum
650 $aDefesa Vegetal
650 $aGlicoproteína
653 $aPeptidogalactomannan
700 1 $aMONTEBIANCO, C.
700 1 $aROMANEL, E.
700 1 $aSILVA, T. da F.
700 1 $aBERNABÉ, R. B.
700 1 $aSIMAS-TOSIN, F.
700 1 $aSOUZA, L. M.
700 1 $aSASSAKI, G. L.
700 1 $aVASLIN, M. F. S.
700 1 $aBARRETO-BERGTER, E.
773 $tPlant Physiology and Biochemistry$gv. 126, p. 206-216, May 2018.
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| Registros recuperados : 108 | |
| 101. |  | HARTERREITEN-SOUZA, E. S.; SOUZA, H. H. S. de; BRAVO, L. K. B.; OLIVEIRA, A. R.; MADEIRA, L. F.; LOPES, L. V.; SOUZA, A. E. R. A.; TOGNI, P. H. B.; SOUSA, A. A. T. C.; SOUZA, L. M. de; PIRES, C. S. S.; PUJOL-LUZ, J. R.; SUJII, E. R. Área de pousio como hábitat fonte para manutenção de moscas predadoras e parasitoides em agroecossistemas de hortaliças. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 102. | | SOUZA, L. M. de; LE GUEN, V.; CERQUEIRA-SILVA, C. B. M.; SILVA, C. C.; MANTELLO, C. C.; CONSON, A. R. O.; VIANNA, J. P. G.; ZUCCHI, M. I.; SCALOPPI JUNIOR, E. J.; FIALHO, J. de F.; MORAES, M. L. T. de; GONÇALVES, P. de S.; SOUZA, A. P. de. Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-Genotype core collection. Plos One, San Francisco, v. 10, n. 7, e013607, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 103. | | BRAVO, L. K. B.; OLIVEIRA, A. R.; TOGNI, P. H. B.; SOUZA, A. E. R. A.; MADEIRA, L. F.; LOPES, L. V.; PIMENTA, M.; HARTERREITEN-SOUZA, E. S.; SOUSA, A. A. T. C. de; SOUZA, L. M. de; PIRES, C. S. S.; SUJII, E. R. Influência da Harmonia axyridis uso de habitat por Cycloneda sanguinea em sistemas orgânicos. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 104. | | SOUZA, L. M. de; SANTOS, L. H. B. dos; ROSA, J. R. B. F.; SILVA, C. C. da; MANTELLO, C. C.; CONSON, A. R. O.; SCALOPPI JUNIOR, E. J.; FIALHO, J. de F.; MORAES, M. L. T. de; GONÇALVES, P. de S.; MARGARIDO, G. R. A.; GARCIA, A. A. F.; LE GUEN, V.; SOUZA, A. P. de. Linkage disequilibrium and population structure in wild and cultivated populations of rubber tree (Hevea brasiliensis). Frontiers in Plant Science, v. 9, article 815, 03 July 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 105. | | OLIVEIRA, A. R.; BRAVO, L. K. B.; SOUZA, A. E. R. A.; TOGNI, P. H. B.; LOPES, L. V.; MADEIRA, L. F.; SOARES, H. H.; PIMENTA, M.; SOUZA, L. M. de; HARTERREITEN-SOUZA, E. S.; SOUSA, A. A. T. C. de; PIRES, C. S. S.; SUJII, E. R. Estrutura da comunidade de Coccinellidae em sistemas orgânicos de produção de hortaliças In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 106. | | SOUZA, L. M. de; ROSSI, M. B.; TOREZANI, K. R. de S.; SOUZA, A. E. R. de A.; HARTERREITEN-SOUZA, E. S.; FRIZZO, T. L. M.; AQUINO, M. F. S. de; TOGNI, P. H. B.; SCHMIDT, F. G. V.; LAUMANN, R. A.; SOUSA, A. A. T. C. de; FONTES, E. M. G.; PIRES, C. S. S.; SUJII, E. R. Coleção entomológica de trabalho do Laboratório de Ecologia e Biossegurança da Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2015. 28 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 349).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 107. | | LOPES, L. V.; PIMENTA, M.; SOUZA, A. E. R. A.; TOGNI, P. H. B.; SOUSA, A. A. T. C. de; SOUZA, L. M. de; BRAVO, L. K. B.; OLIVEIRA, A. R.; MADEIRA, L. F.; PIRES, C. S. S.; SUJII, E. R. Diversidade de Reduviidae (Heteroptera) em cultivos orgânicos de hortaliças. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 108. | | MADEIRA, L. F.; SOUZA, A. E. R. A.; LOPES, L. V.; BRAVO, L. K. B.; OLIVEIRA, A. R.; TOGNI, P. H. B.; HARTERREITEN-SOUZA, E. S.; SOUSA, A. A. T. C. de; SOUZA, L. M. de; PIRES, C. S. S.; SUJII, E. R. Uso de habitat por Chrysoperla externa (Neuroptera: Chrysopidae) em sistemas orgânicos de produção de hortaliças. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 108 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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