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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2010 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, I.; SILVA, P. V.; LANGE, C. C.; GUIMARAES, M. F. M.; WELLER, M. M. D. C. A.; SOUSA, K. R. S.; LOPES, P. S.; GUIMARÃES, J. D.; GUIMARÃES, S. E. F. |
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Afiliação: |
ISABELA FONSECA, UFV; PRISCILA VENDRAMINI SILVA, UFV; CARLA CHRISTINE LANGE, CNPGL; MARTA FONSECA MARTINS GUIMARAES, CNPGL; MAYARA MORENA DEL CAMBRE AMARAL WELLER, UFV; KATIENE RÉGIA SILVA SOUSA, UFV; PAULO SÁVIO LOPES, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE E. F. GUIMARÃES, UFV. |
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Título: |
Expression profile of genes associated with mastitis in dairy cattle. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009. |
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DOI: |
https://doi.org/10.1590/S1415-47572009005000074 |
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Idioma: |
Inglês |
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Conteúdo: |
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis. |
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Palavras-Chave: |
Cytokine; Real-time PCR. |
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Thesaurus Nal: |
gene expression; immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23937/1/Lange-2009-GenMolBiol.pdf
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Marc: |
LEADER 02177naa a2200277 a 4500
001 1697368
005 2024-02-05
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1415-47572009005000074$2DOI
100 1 $aFONSECA, I.
245 $aExpression profile of genes associated with mastitis in dairy cattle.$h[electronic resource]
260 $c2009
520 $aIn order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.
650 $agene expression
650 $aimmune response
653 $aCytokine
653 $aReal-time PCR
700 1 $aSILVA, P. V.
700 1 $aLANGE, C. C.
700 1 $aGUIMARAES, M. F. M.
700 1 $aWELLER, M. M. D. C. A.
700 1 $aSOUSA, K. R. S.
700 1 $aLOPES, P. S.
700 1 $aGUIMARÃES, J. D.
700 1 $aGUIMARÃES, S. E. F.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 32, n. 4, p. 776-781, 2009.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N; MACHADO, M. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; VIANA, J. H. M. |
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Afiliação: |
Wohlres-Viana S., UFJF; Arashiro E. K. N., UNIV. FEDERAL DE ALFENAS; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; M. P. PALHÃO, UNIV. FEDERAL DE ALFENAS; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Reproduction Fertility and Development, v. 29, n. 10, p. 1958-1970, 2017. |
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DOI: |
10.1071/RD16284 |
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Idioma: |
Inglês |
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Notas: |
Publicado online em 19 nov. 2016. |
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Conteúdo: |
Abstract The aim of the present study was to characterise the roles of intrafollicular oestradiol production and granulosa cell (GC) expression of the LH receptor (LHR) gene and its isoforms during follicular deviation in Bos indicus. Follicular wave emergence was synchronised in heifers from a Bos taurus dairy (Holstein; n=10) and a B. indicus dairy breed (Gir; n=10). Follicles were aspirated individually at sizes corresponding to the periods of predeviation, deviation and postdeviation. Intrafollicular oestradiol (IF-E2) and progesterone (IF-P4) concentrations were determined in the follicular fluid (FF) by radioimmunoassay, and relative expression of P450 aromatase (CYP19A1) and LHR forms was evaluated in GC using real-time quantitative-polymerase chain reaction. Despite differences in the size of the dominant follicle at deviation, changes in CYP19A1 expression and IF-E2 concentrations were similar in follicles of the same diameter in both breeds. A peak in total LHR expression occurred after follicular deviation in association with low expression of LHR isoforms. The results suggest that regulation of LHR function by sequential changes in the expression pattern of LHR isoforms may play a role in the early deviation of the dominant follicle, as observed in B. indicus breeds. |
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Palavras-Chave: |
Bovine; Follicular dynamics. |
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Thesagro: |
Bos Indicus. |
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Thesaurus NAL: |
Alternative splicing; Steroidogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02210naa a2200277 a 4500
001 2064888
005 2023-01-30
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1071/RD16284$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aIntrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus.$h[electronic resource]
260 $c2017
500 $aPublicado online em 19 nov. 2016.
520 $aAbstract The aim of the present study was to characterise the roles of intrafollicular oestradiol production and granulosa cell (GC) expression of the LH receptor (LHR) gene and its isoforms during follicular deviation in Bos indicus. Follicular wave emergence was synchronised in heifers from a Bos taurus dairy (Holstein; n=10) and a B. indicus dairy breed (Gir; n=10). Follicles were aspirated individually at sizes corresponding to the periods of predeviation, deviation and postdeviation. Intrafollicular oestradiol (IF-E2) and progesterone (IF-P4) concentrations were determined in the follicular fluid (FF) by radioimmunoassay, and relative expression of P450 aromatase (CYP19A1) and LHR forms was evaluated in GC using real-time quantitative-polymerase chain reaction. Despite differences in the size of the dominant follicle at deviation, changes in CYP19A1 expression and IF-E2 concentrations were similar in follicles of the same diameter in both breeds. A peak in total LHR expression occurred after follicular deviation in association with low expression of LHR isoforms. The results suggest that regulation of LHR function by sequential changes in the expression pattern of LHR isoforms may play a role in the early deviation of the dominant follicle, as observed in B. indicus breeds.
650 $aAlternative splicing
650 $aSteroidogenesis
650 $aBos Indicus
653 $aBovine
653 $aFollicular dynamics
700 1 $aARASHIRO, E. K. N
700 1 $aMACHADO, M. A.
700 1 $aCAMARGO, L. S. de A.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aPALHÃO, M. P.
700 1 $aVIANA, J. H. M.
773 $tReproduction Fertility and Development$gv. 29, n. 10, p. 1958-1970, 2017.
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Embrapa Gado de Leite (CNPGL) |
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