02210naa a2200277 a 450000100080000000500110000800800410001902400250006010000220008524501720010726000090027950000380028852013040032665000250163065000200165565000160167565300110169165300240170270000220172670000190174870000250176770000230179270000190181570000200183477300780185420648882023-01-30       2017    bl uuuu     u00u1 u    #d7 a10.1071/RD162842DOI1 aWOHLRES-VIANA, S.  aIntrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus.h[electronic resource]  c2017  aPublicado online em 19 nov. 2016.  aAbstract The aim of the present study was to characterise the roles of intrafollicular oestradiol production and granulosa cell (GC) expression of the LH receptor (LHR) gene and its isoforms during follicular deviation in Bos indicus. Follicular wave emergence was synchronised in heifers from a Bos taurus dairy (Holstein; n=10) and a B. indicus dairy breed (Gir; n=10). Follicles were aspirated individually at sizes corresponding to the periods of predeviation, deviation and postdeviation. Intrafollicular oestradiol (IF-E2) and progesterone (IF-P4) concentrations were determined in the follicular fluid (FF) by radioimmunoassay, and relative expression of P450 aromatase (CYP19A1) and LHR forms was evaluated in GC using real-time quantitative-polymerase chain reaction. Despite differences in the size of the dominant follicle at deviation, changes in CYP19A1 expression and IF-E2 concentrations were similar in follicles of the same diameter in both breeds. A peak in total LHR expression occurred after follicular deviation in association with low expression of LHR isoforms. The results suggest that regulation of LHR function by sequential changes in the expression pattern of LHR isoforms may play a role in the early deviation of the dominant follicle, as observed in B. indicus breeds.  aAlternative splicing  aSteroidogenesis  aBos Indicus  aBovine  aFollicular dynamics1 aARASHIRO, E. K. N1 aMACHADO, M. A.1 aCAMARGO, L. S. de A.1 aSIQUEIRA, L. G. B.1 aPALHÃO, M. P.1 aVIANA, J. H. M.  tReproduction Fertility and Developmentgv. 29, n. 10, p. 1958-1970, 2017.