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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
26/03/1996 |
Data da última atualização: |
05/12/2023 |
Autoria: |
McNAMARA, P. J.; BRADLY, G. A.; SONGER, J. G. |
Título: |
Targeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Molecular Microbiologia, v. 12, n. 6, p. 921-930, Jun. 1994. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host. |
Palavras-Chave: |
Corybacterium pseudotuberculosis; Microbiologia molecular. |
Thesagro: |
Corynebacterium Pseudotuberculosis; Linfadenite Caseosa. |
Thesaurus Nal: |
Amino acid sequences; Enzymology; Gene targeting; Goat diseases; Goats; Microbiology; Mutagenesis; Mutation; Phospholipase D; Physiology; Virulence. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01676naa a2200325 a 4500 001 1522010 005 2023-12-05 008 1994 bl uuuu u00u1 u #d 100 1 $aMcNAMARA, P. J. 245 $aTargeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis.$h[electronic resource] 260 $c1994 520 $aAbstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host. 650 $aAmino acid sequences 650 $aEnzymology 650 $aGene targeting 650 $aGoat diseases 650 $aGoats 650 $aMicrobiology 650 $aMutagenesis 650 $aMutation 650 $aPhospholipase D 650 $aPhysiology 650 $aVirulence 650 $aCorynebacterium Pseudotuberculosis 650 $aLinfadenite Caseosa 653 $aCorybacterium pseudotuberculosis 653 $aMicrobiologia molecular 700 1 $aBRADLY, G. A. 700 1 $aSONGER, J. G. 773 $tMolecular Microbiologia$gv. 12, n. 6, p. 921-930, Jun. 1994.
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