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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
26/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R. |
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Afiliação: |
JULIANA CARINE GERN, CNPGL; LEANDRO FREIRE dos SANTOS, Universidade Federal do Paraná; SASCHA HABU, Universidade Federal do Paraná; USP; MIGUEL DANIEL NOSEDA, Universidade Federal do Paraná; LUCIANNA FREITAS OLIVEIRA de LIMA, Universidade Federal do Paraná; ANDRÉ LUÍS LOPES da SILVA, Universidade Federal do Paraná; VANETE THOMAZ SOCCOL, Universidade Federal do Paraná; HUMBERTO DE MELLO BRANDAO, CNPGL; CARLOS RICARDO SOCCOL, Universidade Federal do Paraná. |
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Título: |
Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Romanian Biotechnological Letters, v. 20, n. 6, p. 10945-10950, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield. |
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Palavras-Chave: |
Agaricus brasiliensis; Basidiomycete; Exopolysaccharide; Monosaccharide composition; Scale up; Stirred tank fermenter. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139533/1/Cnpgl-2015-RomBioLet-Exopolysaccharide.pdf
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Marc: |
LEADER 02098naa a2200289 a 4500
001 2037830
005 2023-06-26
008 2015 bl uuuu u00u1 u #d
100 1 $aGERN, J. C.
245 $aExopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter.$h[electronic resource]
260 $c2015
520 $aBackground and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield.
653 $aAgaricus brasiliensis
653 $aBasidiomycete
653 $aExopolysaccharide
653 $aMonosaccharide composition
653 $aScale up
653 $aStirred tank fermenter
700 1 $aSANTOS, L. F. dos
700 1 $aHABU, S.
700 1 $aNOSEDA, M. D.
700 1 $aLIMA, L. F. O. de
700 1 $aSILVA, A. L. L. da
700 1 $aSOCCOL, V. T.
700 1 $aBRANDAO, H. de M.
700 1 $aSOCCOL, C. R.
773 $tRomanian Biotechnological Letters$gv. 20, n. 6, p. 10945-10950, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
20/04/2023 |
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Data da última atualização: |
15/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CATUNDA, L. G. S.; PRADO, T. M. do; OLIVEIRA, T. R. de; SANTOS, D. J. A. dos; GOMES, N. O.; CORREA, D. S.; FARIA, R. C.; MACHADO, S. A. S. |
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Afiliação: |
DANIEL SOUZA CORREA, CNPDIA. |
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Título: |
SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Electrochimica Acta, v. 451, 142271, 2023. |
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Páginas: |
10 p. |
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ISSN: |
0013-4686 |
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DOI: |
https://doi.org/10.1016/j.electacta.2023.142271 |
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Idioma: |
Inglês |
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Conteúdo: |
Fast, sensitive, simple, and cheap sensors are highly desirable to be applied in the health system because they improve point-of-care diagnostics, which can reduce the number of cases of infection or even deaths. In this context, here we report the development of a label-free genosensor using a screen-printed electrode modified with 2D-carbonylated graphitic carbon nitride (c-g-C3N4), poly(diallyldimethylammonium) chloride (PDDA), and glutathione-protected gold nanoparticles (GSH-AuNPs) for photoelectrochemical (PEC) detection of SARS-CoV-2. We also made use of Arduino and 3D printing to miniaturize the sensor device. The electrode surface was characterized by AFM and SEM techniques, and the gold nanoparticles by UV?Vis spectrophotometry. For SARSCoV-2 detection, capture probe DNA was immobilized on the electrode surface. The hybridization of the final genosensor was tested with a synthetic single-strand DNA target and with natural saliva samples using the photoelectrochemistry method. The device presented a linear range from 1 to 10,000 fmol L− 1 and a limit of detection of 2.2 and 3.4 fmol L− 1 using cpDNA 1A and 3A respectively. The sensibility and accuracy found for the genosensor using cpDNA 1A using biological samples were 93.3 and 80% respectively, indicating the potential of the label-free and portable genosensor to detect SARS-CoV-2 RNA in saliva samples. |
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Palavras-Chave: |
Arduino; Saliva test; Saliva test Miniaturized platform; SARS-CoV-2 detection; SARS-CoV-2 detection Photoelectrochemical (PEC) sensor. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02388naa a2200301 a 4500
001 2153291
005 2024-01-15
008 2023 bl uuuu u00u1 u #d
022 $a0013-4686
024 7 $ahttps://doi.org/10.1016/j.electacta.2023.142271$2DOI
100 1 $aCATUNDA, L. G. S.
245 $aSARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles.$h[electronic resource]
260 $c2023
300 $a10 p.
520 $aFast, sensitive, simple, and cheap sensors are highly desirable to be applied in the health system because they improve point-of-care diagnostics, which can reduce the number of cases of infection or even deaths. In this context, here we report the development of a label-free genosensor using a screen-printed electrode modified with 2D-carbonylated graphitic carbon nitride (c-g-C3N4), poly(diallyldimethylammonium) chloride (PDDA), and glutathione-protected gold nanoparticles (GSH-AuNPs) for photoelectrochemical (PEC) detection of SARS-CoV-2. We also made use of Arduino and 3D printing to miniaturize the sensor device. The electrode surface was characterized by AFM and SEM techniques, and the gold nanoparticles by UV?Vis spectrophotometry. For SARSCoV-2 detection, capture probe DNA was immobilized on the electrode surface. The hybridization of the final genosensor was tested with a synthetic single-strand DNA target and with natural saliva samples using the photoelectrochemistry method. The device presented a linear range from 1 to 10,000 fmol L− 1 and a limit of detection of 2.2 and 3.4 fmol L− 1 using cpDNA 1A and 3A respectively. The sensibility and accuracy found for the genosensor using cpDNA 1A using biological samples were 93.3 and 80% respectively, indicating the potential of the label-free and portable genosensor to detect SARS-CoV-2 RNA in saliva samples.
653 $aArduino
653 $aSaliva test
653 $aSaliva test Miniaturized platform
653 $aSARS-CoV-2 detection
653 $aSARS-CoV-2 detection Photoelectrochemical (PEC) sensor
700 1 $aPRADO, T. M. do
700 1 $aOLIVEIRA, T. R. de
700 1 $aSANTOS, D. J. A. dos
700 1 $aGOMES, N. O.
700 1 $aCORREA, D. S.
700 1 $aFARIA, R. C.
700 1 $aMACHADO, S. A. S.
773 $tElectrochimica Acta$gv. 451, 142271, 2023.
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Embrapa Instrumentação (CNPDIA) |
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