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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/12/2012 |
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Data da última atualização: |
10/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DANES, M. A. C.; CHAGAS, L. J.; SANTOS, F. A. P.; PEDROSO, A. M. |
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Afiliação: |
MARINA A. C. DANES, USP-ESALQ; LUCAS L. CHAGAS, USP-ESALQ; FLAVIO AUGUSTO PORTELA SANTOS, USP-ESALQ; ALEXANDRE MENDONCA PEDROSO, CPPSE. |
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Título: |
Effect of protein supplementation on milk production and metabolism of dairy cows grazing tropical grass. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 96, n. 1, p. 1-13, nov. 2012. |
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DOI: |
10.3168/jds.2012-5607 |
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Idioma: |
Português |
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Conteúdo: |
The objectives of this study were to determine if midlactation dairy cows (Bos taurus L.) grazing intensively managed elephantgrass would have their protein requirement met exclusively with the pasture and an energy concentrate, making the use of protein ingredients unnecessary, as well as to determine the dietary crude protein (CP) content that would optimize the efficiency of N utilization (ENU). Thirty-three Holstein and crossbred (Holstein × Jersey) midlactation dairy cows, producing approximately 20 kg/d, were grouped within breed into 11 blocks according to milk yield and days in milk. Within blocks, cows were randomly assigned to 1 of 3 treatments and remained in the study for 11 wk. The control treatment contained only finely ground corn, minerals, and vitamins, and it was formulated to be 8.7% CP. Two higher levels of CP (formulated to be 13.4 and 18.1%) were achieved by replacing corn with solvent-extracted soybean meal (SSBM). Pasture was fertilized with 50 kg of N/ha after each grazing cycle and averaged 18.5% CP (dry matter basis). No differences were observed in milk yield or milk fat, protein, and casein content or casein yield. In addition, pasture intake was not different among treatments. Milk urea N increased linearly as the concentrate CP content increased. Cows fed the 8.7% CP concentrate had higher ENU. In another experiment, 4 ruminally cannulated Holstein dry cows were used in a metabolism trial designed in a 4 × 4 Latin square. Cows were fed the same treatments described as well as a fourth treatment with 13.4% CP in the concentrate, in which urea replaced SSBM as the main N source. Ruminal volatile fatty acid concentration and microbial synthesis were not affected by levels or sources of N in the concentrate. Ruminal NH3N content increased as the concentrate CP content increased. Inclusion of SSBM in the concentrate did not increase production and decreased the ENU of midlactation dairy cows grazing on tropical forage. Supplementation of an 8.7% CP concentrate, resulting in a diet with CP levels between 15.3 and 15.7% of dry matter, was sufficient to meet the protein requirements of such milk production, with the highest ENU (18.4%). MenosThe objectives of this study were to determine if midlactation dairy cows (Bos taurus L.) grazing intensively managed elephantgrass would have their protein requirement met exclusively with the pasture and an energy concentrate, making the use of protein ingredients unnecessary, as well as to determine the dietary crude protein (CP) content that would optimize the efficiency of N utilization (ENU). Thirty-three Holstein and crossbred (Holstein × Jersey) midlactation dairy cows, producing approximately 20 kg/d, were grouped within breed into 11 blocks according to milk yield and days in milk. Within blocks, cows were randomly assigned to 1 of 3 treatments and remained in the study for 11 wk. The control treatment contained only finely ground corn, minerals, and vitamins, and it was formulated to be 8.7% CP. Two higher levels of CP (formulated to be 13.4 and 18.1%) were achieved by replacing corn with solvent-extracted soybean meal (SSBM). Pasture was fertilized with 50 kg of N/ha after each grazing cycle and averaged 18.5% CP (dry matter basis). No differences were observed in milk yield or milk fat, protein, and casein content or casein yield. In addition, pasture intake was not different among treatments. Milk urea N increased linearly as the concentrate CP content increased. Cows fed the 8.7% CP concentrate had higher ENU. In another experiment, 4 ruminally cannulated Holstein dry cows were used in a metabolism trial designed in a 4 × 4 Latin square. Cows were fed the same... Mostrar Tudo |
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Palavras-Chave: |
Protein supplementation; Tropical pasture. |
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Thesaurus Nal: |
nitrogen metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02857naa a2200205 a 4500
001 1942179
005 2023-03-10
008 2012 bl uuuu u00u1 u #d
024 7 $a10.3168/jds.2012-5607$2DOI
100 1 $aDANES, M. A. C.
245 $aEffect of protein supplementation on milk production and metabolism of dairy cows grazing tropical grass.$h[electronic resource]
260 $c2012
520 $aThe objectives of this study were to determine if midlactation dairy cows (Bos taurus L.) grazing intensively managed elephantgrass would have their protein requirement met exclusively with the pasture and an energy concentrate, making the use of protein ingredients unnecessary, as well as to determine the dietary crude protein (CP) content that would optimize the efficiency of N utilization (ENU). Thirty-three Holstein and crossbred (Holstein × Jersey) midlactation dairy cows, producing approximately 20 kg/d, were grouped within breed into 11 blocks according to milk yield and days in milk. Within blocks, cows were randomly assigned to 1 of 3 treatments and remained in the study for 11 wk. The control treatment contained only finely ground corn, minerals, and vitamins, and it was formulated to be 8.7% CP. Two higher levels of CP (formulated to be 13.4 and 18.1%) were achieved by replacing corn with solvent-extracted soybean meal (SSBM). Pasture was fertilized with 50 kg of N/ha after each grazing cycle and averaged 18.5% CP (dry matter basis). No differences were observed in milk yield or milk fat, protein, and casein content or casein yield. In addition, pasture intake was not different among treatments. Milk urea N increased linearly as the concentrate CP content increased. Cows fed the 8.7% CP concentrate had higher ENU. In another experiment, 4 ruminally cannulated Holstein dry cows were used in a metabolism trial designed in a 4 × 4 Latin square. Cows were fed the same treatments described as well as a fourth treatment with 13.4% CP in the concentrate, in which urea replaced SSBM as the main N source. Ruminal volatile fatty acid concentration and microbial synthesis were not affected by levels or sources of N in the concentrate. Ruminal NH3N content increased as the concentrate CP content increased. Inclusion of SSBM in the concentrate did not increase production and decreased the ENU of midlactation dairy cows grazing on tropical forage. Supplementation of an 8.7% CP concentrate, resulting in a diet with CP levels between 15.3 and 15.7% of dry matter, was sufficient to meet the protein requirements of such milk production, with the highest ENU (18.4%).
650 $anitrogen metabolism
653 $aProtein supplementation
653 $aTropical pasture
700 1 $aCHAGAS, L. J.
700 1 $aSANTOS, F. A. P.
700 1 $aPEDROSO, A. M.
773 $tJournal of Dairy Science$gv. 96, n. 1, p. 1-13, nov. 2012.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/09/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LABATE, M. T. V.; Ko, KENTON; Ko, Z. W.; PINTO, L. S. R. C.; REAL, M. J. U. D.; ROMANO, M. R.; BARJA, P. R.; GRANELL, A.; FRISO, G.; WIJK, K. J. V.; BRUGNOLI, E.; LABATE, C. A. |
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Afiliação: |
MÔNICA T. V. LABATE, ESALQ; KENTON KO, Queen’s University; ZDENKA W. Ko, Queen’s University; LUCIANA S. R. COSTA PINTO, ESALQ; MARIA J. U. D. REAL, ESALQ; MARCELO RIBEIRO ROMANO, CNPMF; PAULO ROXO BARJA, ESALQ; ANTONIO GRANELL, Universidad Politecnica de Valencia; GIULIA FRISO, Cornell University; KLAAS J. VAN WIJK, Cornell University; ENRICO BRUGNOLI, Instituto di Biologia Agroambientale; CARLOS A. LABATE, ESALQ. |
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Título: |
Constitutive expression of pea Lhcb1-2 in tobacco affects plant development, morphology and photosynthetic capacity. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology, v. 55, p. 701-714, 2014. |
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ISSN: |
0167-4412 |
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Idioma: |
Inglês |
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Conteúdo: |
Lhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature MenosLhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylako... Mostrar Tudo |
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Palavras-Chave: |
Tabaco. |
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Thesagro: |
Clorofila; Ervilha; Fumo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02643naa a2200313 a 4500
001 1994384
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
022 $a0167-4412
100 1 $aLABATE, M. T. V.
245 $aConstitutive expression of pea Lhcb1-2 in tobacco affects plant development, morphology and photosynthetic capacity.$h[electronic resource]
260 $c2014
520 $aLhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature
650 $aClorofila
650 $aErvilha
650 $aFumo
653 $aTabaco
700 1 $aKo, KENTON
700 1 $aKo, Z. W.
700 1 $aPINTO, L. S. R. C.
700 1 $aREAL, M. J. U. D.
700 1 $aROMANO, M. R.
700 1 $aBARJA, P. R.
700 1 $aGRANELL, A.
700 1 $aFRISO, G.
700 1 $aWIJK, K. J. V.
700 1 $aBRUGNOLI, E.
700 1 $aLABATE, C. A.
773 $tPlant Molecular Biology$gv. 55, p. 701-714, 2014.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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