02857naa a2200205 a 450000100080000000500110000800800410001902400310006010000200009124501330011126000090024452022040025365000240245765300280248165300210250970000180253070000210254870000190256977300630258819421792023-03-10       2012    bl uuuu     u00u1 u    #d7 a10.3168/jds.2012-56072DOI1 aDANES, M. A. C.  aEffect of protein supplementation on milk production and metabolism of dairy cows grazing tropical grass.h[electronic resource]  c2012  aThe objectives of this study were to determine if midlactation dairy cows (Bos taurus L.) grazing intensively managed elephantgrass would have their protein requirement met exclusively with the pasture and an energy concentrate, making the use of protein ingredients unnecessary, as well as to determine the dietary crude protein (CP) content that would optimize the efficiency of N utilization (ENU). Thirty-three Holstein and crossbred (Holstein × Jersey) midlactation dairy cows, producing approximately 20 kg/d, were grouped within breed into 11 blocks according to milk yield and days in milk. Within blocks, cows were randomly assigned to 1 of 3 treatments and remained in the study for 11 wk. The control treatment contained only finely ground corn, minerals, and vitamins, and it was formulated to be 8.7% CP. Two higher levels of CP (formulated to be 13.4 and 18.1%) were achieved by replacing corn with solvent-extracted soybean meal (SSBM). Pasture was fertilized with 50 kg of N/ha after each grazing cycle and averaged 18.5% CP (dry matter basis). No differences were observed in milk yield or milk fat, protein, and casein content or casein yield. In addition, pasture intake was not different among treatments. Milk urea N increased linearly as the concentrate CP content increased. Cows fed the 8.7% CP concentrate had higher ENU. In another experiment, 4 ruminally cannulated Holstein dry cows were used in a metabolism trial designed in a 4 × 4 Latin square. Cows were fed the same treatments described as well as a fourth treatment with 13.4% CP in the concentrate, in which urea replaced SSBM as the main N source. Ruminal volatile fatty acid concentration and microbial synthesis were not affected by levels or sources of N in the concentrate. Ruminal NH3N content increased as the concentrate CP content increased. Inclusion of SSBM in the concentrate did not increase production and decreased the ENU of midlactation dairy cows grazing on tropical forage. Supplementation of an 8.7% CP concentrate, resulting in a diet with CP levels between 15.3 and 15.7% of dry matter, was sufficient to meet the protein requirements of such milk production, with the highest ENU (18.4%).  anitrogen metabolism  aProtein supplementation  aTropical pasture1 aCHAGAS, L. J.1 aSANTOS, F. A. P.1 aPEDROSO, A. M.  tJournal of Dairy Sciencegv. 96, n. 1, p. 1-13, nov. 2012.