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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
18/08/1999 |
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Data da última atualização: |
16/05/2023 |
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Autoria: |
FITCH, M. M. M.; MANSHARDT, R. M.; GONSALVES, D.; SLIGHTOM, J. L.; SANFORD, J. C. |
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Afiliação: |
University of Hawaii; University Geneva; The Upjohn Company, Kalamazoo, MI 49001, USA. |
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Título: |
Stable transformation of papaya via microprojectile bombardment. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Plant Cell Reports, v.9, p.189-194, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Stable transformation of papaya (carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10mg 1-1 2,4-D, 400mg 1-1 glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150mg 1-1 kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots. |
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Thesagro: |
Mamão. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01189naa a2200181 a 4500
001 1648268
005 2023-05-16
008 1990 bl uuuu u00u1 u #d
100 1 $aFITCH, M. M. M.
245 $aStable transformation of papaya via microprojectile bombardment.$h[electronic resource]
260 $c1990
520 $aStable transformation of papaya (carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10mg 1-1 2,4-D, 400mg 1-1 glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150mg 1-1 kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots.
650 $aMamão
700 1 $aMANSHARDT, R. M.
700 1 $aGONSALVES, D.
700 1 $aSLIGHTOM, J. L.
700 1 $aSANFORD, J. C.
773 $tPlant Cell Reports$gv.9, p.189-194, 1990.
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| 1. |  | ARAGÃO, F. J. L.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; RIBEIRO, S. G.; SMITH, F. D.; SANFORD, J. C.; FARIA, J. C.; RECH, E. L. Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theoretical and Applied Genetics, Berlin, v.93, n.l/2, p.142-150, l996. p. 142-150| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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