01189naa a2200181 a 450000100080000000500110000800800410001910000200006024500920008026000090017252006910018165000110087270000210088370000180090470000200092270000190094277300460096116482682023-05-16       1990    bl uuuu     u00u1 u    #d1 aFITCH, M. M. M.  aStable transformation of papaya via microprojectile bombardment.h[electronic resource]  c1990  aStable transformation of papaya (carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10mg 1-1 2,4-D, 400mg 1-1 glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150mg 1-1 kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots.  aMamão1 aMANSHARDT, R. M.1 aGONSALVES, D.1 aSLIGHTOM, J. L.1 aSANFORD, J. C.  tPlant Cell Reportsgv.9, p.189-194, 1990.