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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/10/2011 |
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Data da última atualização: |
13/10/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIOLA, V.; BRITO, G. G. de; CAIXETA, E. T.; MACIEL-ZAMBOLIM, E.; SAKIYAMA, N. S.; LOUREIRO, M. E. |
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Afiliação: |
Universidade Federal Rural do Rio de Janeiro; GIOVANI GREIGH DE BRITO, CNPA; EVELINE TEIXEIRA CAIXETA, SAPC; BIOCAFÉ/BIOAGRO; BIOCAFÉ/BIOAGRO; Universidade Federal de Viçosa. |
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Título: |
High-density genetic mapping for coffee leaf rust resistance. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Tree Genetics & Genomes, v. 7, n. 6, p. 1199-1208, Dec. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control, the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic factors, mainly those found in material originating from interspecific hybrids. In this study, the genetic analysis of an F2 population consisting of 224 plants derived from a crossing of Híbrido de Timor UFV 427-15 (resistant) with Catuaí Amarelo IAC 30 (susceptible) showed that a dominant gene confers the resistance of coffee to race II of H. vastatrix. From a genetic map saturated with 25 amplified fragment length polymorphism (AFLP) markers linked to the resistance gene, we developed a high-density genetic map with six sequence-characterized amplified region (SCAR) markers delimiting a chromosomal region of 9.45 cM and flanking the dominant gene at 0.7 and 0.9 cM. This is the first saturated and high-density genetic map obtained from this region containing the resistance gene. The results of this study are of great importance for the introduction of molecular markers for marker-assisted selection; they will also facilitate studies related to the cloning, structure, and function of race-specific genes involved in the resistance of coffee trees to H. vastatrix. |
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Palavras-Chave: |
Dominant resistance gene; High-density genetic map. |
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Thesagro: |
Coffea Arábica; Hemileia Vastatrix. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42966/1/High-density-genetic.pdf
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Marc: |
LEADER 02058naa a2200229 a 4500
001 1902620
005 2016-10-13
008 2011 bl uuuu u00u1 u #d
100 1 $aDIOLA, V.
245 $aHigh-density genetic mapping for coffee leaf rust resistance.$h[electronic resource]
260 $c2011
520 $aCoffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control, the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic factors, mainly those found in material originating from interspecific hybrids. In this study, the genetic analysis of an F2 population consisting of 224 plants derived from a crossing of Híbrido de Timor UFV 427-15 (resistant) with Catuaí Amarelo IAC 30 (susceptible) showed that a dominant gene confers the resistance of coffee to race II of H. vastatrix. From a genetic map saturated with 25 amplified fragment length polymorphism (AFLP) markers linked to the resistance gene, we developed a high-density genetic map with six sequence-characterized amplified region (SCAR) markers delimiting a chromosomal region of 9.45 cM and flanking the dominant gene at 0.7 and 0.9 cM. This is the first saturated and high-density genetic map obtained from this region containing the resistance gene. The results of this study are of great importance for the introduction of molecular markers for marker-assisted selection; they will also facilitate studies related to the cloning, structure, and function of race-specific genes involved in the resistance of coffee trees to H. vastatrix.
650 $aCoffea Arábica
650 $aHemileia Vastatrix
653 $aDominant resistance gene
653 $aHigh-density genetic map
700 1 $aBRITO, G. G. de
700 1 $aCAIXETA, E. T.
700 1 $aMACIEL-ZAMBOLIM, E.
700 1 $aSAKIYAMA, N. S.
700 1 $aLOUREIRO, M. E.
773 $tTree Genetics & Genomes$gv. 7, n. 6, p. 1199-1208, Dec. 2011.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
05/11/2020 |
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Data da última atualização: |
05/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOARES, S. L.; ANCIUTIA, A. N.; DIAS, L.; CORCINI, C. D.; VARELA JÚNIOR, A. S.; KOMNINOU, E. R.; TEBALDI, M. L.; MARQUES, M. G.; FONSECA, F. M. da; LUCIA JUNIOR, T. |
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Afiliação: |
SARA L. SOARES, UFPel; ANDREIA N. ANCIUTI, UFPel; LUCIA DIAS, UFPel; CARINE DAHL CORCINI, UFPel; ANTÔNIO S. VARELA JÚNIOR, FURG; ELIZA R. KOMNINOU, UFPel; MARLI LUIZA TEBALDI, UNIFEI; MARIANA GROKE MARQUES, CNPSA; FRANCISCO NOE DA FONSECA, CNPSA; THOMAZ LUCIA JUNIOR, UFPel. |
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Título: |
Safety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Toxicology in Vitro, v. 65, n. 104766, 2020. |
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DOI: |
https://doi.org/10.1016/j.tiv.2020.104766 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 μg/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P > .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P > .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P > .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P > .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders. |
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Thesagro: |
Criopreservação; Espermatozóide; Javali; Reprodução Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02092naa a2200289 a 4500
001 2126319
005 2020-11-05
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.tiv.2020.104766$2DOI
100 1 $aSOARES, S. L.
245 $aSafety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation.$h[electronic resource]
260 $c2020
520 $aAbstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 μg/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P > .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P > .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P > .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P > .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders.
650 $aCriopreservação
650 $aEspermatozóide
650 $aJavali
650 $aReprodução Animal
700 1 $aANCIUTIA, A. N.
700 1 $aDIAS, L.
700 1 $aCORCINI, C. D.
700 1 $aVARELA JÚNIOR, A. S.
700 1 $aKOMNINOU, E. R.
700 1 $aTEBALDI, M. L.
700 1 $aMARQUES, M. G.
700 1 $aFONSECA, F. M. da
700 1 $aLUCIA JUNIOR, T.
773 $tToxicology in Vitro$gv. 65, n. 104766, 2020.
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