02092naa a2200289 a 450000100080000000500110000800800410001902400510006010000180011124501480012926000090027752012110028665000220149765000200151965000110153965000240155070000200157470000130159470000190160770000260162670000200165270000190167270000190169170000220171070000210173277300490175321263192020-11-05       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.tiv.2020.1047662DOI1 aSOARES, S. L.  aSafety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation.h[electronic resource]  c2020  aAbstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 μg/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P &gt .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P &gt .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P &gt .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P &gt .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders.  aCriopreservação  aEspermatozóide  aJavali  aReprodução Animal1 aANCIUTIA, A. N.1 aDIAS, L.1 aCORCINI, C. D.1 aVARELA JÚNIOR, A. S.1 aKOMNINOU, E. R.1 aTEBALDI, M. L.1 aMARQUES, M. G.1 aFONSECA, F. M. da1 aLUCIA JUNIOR, T.  tToxicology in Vitrogv. 65, n. 104766, 2020.