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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
29/05/2023 |
Data da última atualização: |
25/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
Título: |
A novel soybean hairy root system for gene functional validation. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
Idioma: |
Inglês |
Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
Thesaurus Nal: |
Soybeans. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
LEADER 02598naa a2200337 a 4500 001 2154066 005 2024-04-25 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI 100 1 $aPEREIRA, B. M. 245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource] 260 $c2023 500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. 520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. 650 $aSoybeans 650 $aGlycine Max 650 $aMelhoramento Genético Vegetal 650 $aMeloidogyne spp 650 $aSoja 653 $aAgrobacterium rhizogenes 653 $aTransgenic soybean 700 1 $aARRAES, F. 700 1 $aMARTINS, A. C. Q. 700 1 $aALVES, N. S. F. 700 1 $aMELO, B. P. 700 1 $aMORGANTE, C. V. 700 1 $aSARAIVA, M. A. P. 700 1 $aSA, M. F. G. de 700 1 $aGUIMARAES, P. M. 700 1 $aBRASILEIRO, A. C. M. 773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registros recuperados : 222 | |
141. | | FAZOLIN, M.; ESTRELA, J. L. V.; CATANI, V.; ALÉCIO, M. R.; LIMA, M. S. de. Propriedade inseticida dos óleos essenciais de Piper hispidinervum C. DC.; Piper aduncum L. e Tanaecium nocturnum (Barb. Rodr.) Bur. & K. Shum sobre Tenebrio molitor L., 1758. Ciência e Agrotecnologia, Lavras, v. 31, n. 1, p. 113-120, jan./fev. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Embrapa Acre. |
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147. | | BOIÇA JÚNIOR, A. L.; MATIOLI, J. C.; FAZOLIN, M.; NAKANO, O.; RIVERO, R. C. Difusão de fosfina no interior de cupinzeiros de Cornitermes cumulans (Kollar) (Isoptera: Termitidae). Anais da Sociedade Entomologica do Brasil, Londrina, v. 21, n. 3, p. 307-317, 1992.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
Biblioteca(s): Embrapa Acre. |
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152. | | NORONHA, A. C. da S.; LEMOS, W. de P.; FAZOLIN, M.; SANCHES, N. F.; GARCIA, M. V. B. Abacaxi. In: SILVA, N. M. da; ADAIME, R.; ZUCCHI, R. A. (ed.). Pragas agrícolas e florestais na Amazônia. Brasília, DF: Embrapa, 2016. pt. 1, cap. 1, p. 23-43.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Acre; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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154. | | SIHLER, W.; SANCHES, M. M.; FALCAO, R.; FAZOLIN, M.; ESTRELA, J. L. V.; SOUZA, M. L. de. Caracterização de Erinnyis ello Granulovirus isolado de populações de mandarová da mandioca em Cruzeiro do Sul, Acre. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. Goiânia: Sociedade Entomológica do Brasil, 2014. Resumo 1102.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Acre; Embrapa Recursos Genéticos e Biotecnologia. |
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157. | | FAZOLIN, M.; FAZOLIN, J. L. V.; CATANI, V.; LIMA, M. S. de; ALÉCIO, M. R.; COSTA, C. R. da. Inseticidas botânicos da flora amazônica: potencial inseticida de óleos essenciais no controle de Tenebrio molitor L., 1758. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 3., 2006, Belém, PA. Anais...Belém: Embrapa Amazônia Oriental: Sebrae, 2006. R. 39. p. 30.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Acre. |
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158. | | FAZOLIN, M.; COSTA, C. R. da; ESTRELA, J. L. V.; HESSEL, C. E.; ANDRADE, C. M. S. de. Levantamento de insetos-praga associados aos capins tanner-grass, tangola e estrela-africana no Acre. Amazônia: Ciência & Desenvolvimento, Belém, v. 4, n. 8, p. 273-283, jan./jun. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
Biblioteca(s): Embrapa Acre. |
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159. | | SILVIE, P.; FAZOLIN, M.; RIBEIRO, L. do P.; MARCHAND, P. A.; TCHUWA, F.; MARTIN, P.; MKINDI, A. The long and challenging road to capitalize on plant-based extracts from the lab to the field. In: EUROPEAN CONGRESS OF ENTOMOLOGY, 12., 2023, Crete, Greece. Book of abstracts... Crete, Greece: Hellenic Entomological Society, 2023. p. 334.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Acre. |
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160. | | FAZOLIN, M.; BIZZO, H. R.; MONTEIRO, A. F. M.; LIMA, M. E. C.; MAISFORTE, N. S.; GAMA, P. E. Synergism in two-component insecticides with dillapiole against fall armyworm. Plants, v. 12, n. 17, 3042, Sept. 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
Biblioteca(s): Embrapa Acre; Embrapa Agroindústria de Alimentos. |
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Registros recuperados : 222 | |
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