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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
26/05/2023 |
Data da última atualização: |
29/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FONSECA, F. C. de A.; ANTONINO, J. D.; MOURA, S. M. de; RODRIGUES-SILVA, P. L.; MACEDO, L. L. P. de; GOMES JÚNIOR, J. E.; LOURENCO, I. T.; LUCENA, W. A.; MORGANTE, C. V.; RIBEIRO, T. P.; MONNERAT, R. G.; RODRIGUES, M. A.; CUCCOVIA, I. M.; SILVA, M. C. M. da; SA, M. F. G. de. |
Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, University of Brasília; JOSÉ DIJAIR ANTONINO, University of Brasília; STÉFANIE MENEZES DE MOURA, National Institute of Science and Technology; PAOLO LUCAS RODRIGUES-SILVA, National Institute of Science and Technology; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; JOSÉ EDÍLSON GOMES JÚNIOR, Federal University of Brasília; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; WAGNER ALEXANDRE LUCENA, ARIG; CAROLINA VIANNA MORGANTE, CPATSA; THUANNE PIRES RIBEIRO, National Institute of Science and Technology; ROSE GOMES MONNERAT; MAGALI APARECIDA RODRIGUES, 7University of São Paulo; IOLANDA MIDEA CUCCOVIA, University of São Paulo; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Bulletin of Entomological Research, v. 113, n. 3, p. 335-346, 2023. |
DOI: |
https://doi.org/10.1017/S000748532200061X |
Idioma: |
Inglês |
Notas: |
Na publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
Conteúdo: |
The sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields. MenosThe sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic s... Mostrar Tudo |
Palavras-Chave: |
Aminopeptidase N; Broca gigante da cana-de-açúcar; Cry toxins; Modelagem molecular; Molecular modeling; Techin licus licus; Toxinas Cry. |
Thesagro: |
Bacillus Thuringiensis; Cana de Açúcar; Inseto; Praga; Saccharum Officinarum. |
Thesaurus Nal: |
Sugarcane. |
Categoria do assunto: |
-- O Insetos e Entomologia |
Marc: |
LEADER 03134naa a2200469 a 4500 001 2154056 005 2023-05-29 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S000748532200061X$2DOI 100 1 $aFONSECA, F. C. de A. 245 $aIn vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer.$h[electronic resource] 260 $c2023 500 $aNa publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. 520 $aThe sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields. 650 $aSugarcane 650 $aBacillus Thuringiensis 650 $aCana de Açúcar 650 $aInseto 650 $aPraga 650 $aSaccharum Officinarum 653 $aAminopeptidase N 653 $aBroca gigante da cana-de-açúcar 653 $aCry toxins 653 $aModelagem molecular 653 $aMolecular modeling 653 $aTechin licus licus 653 $aToxinas Cry 700 1 $aANTONINO, J. D. 700 1 $aMOURA, S. M. de 700 1 $aRODRIGUES-SILVA, P. L. 700 1 $aMACEDO, L. L. P. de 700 1 $aGOMES JÚNIOR, J. E. 700 1 $aLOURENCO, I. T. 700 1 $aLUCENA, W. A. 700 1 $aMORGANTE, C. V. 700 1 $aRIBEIRO, T. P. 700 1 $aMONNERAT, R. G. 700 1 $aRODRIGUES, M. A. 700 1 $aCUCCOVIA, I. M. 700 1 $aSILVA, M. C. M. da 700 1 $aSA, M. F. G. de 773 $tBulletin of Entomological Research$gv. 113, n. 3, p. 335-346, 2023.
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2. | | LOPES, P. V. L.; CERQUEIRA, R. C.; SOARES NETO, J. P.; DUSI, A. N.; RESENDE, F. V. Efeito da qualidade da semente na produção de alho no oeste baiano. Horticultura Brasileira, Brasília, DF, v. 24, n. 1, jul. 2006. Suplemento 1. Resumo 225. Trabalho apresentado no 46. Congresso Brasileiro de Olericultura, Goiânia, 2006. Publicado também como resumo expandido na Horticultura Brasileira, Brasília, DF, v. 24, n. 2, p. 889, jul. 2006. Suplemento 2. CD-ROM.Biblioteca(s): Embrapa Hortaliças. |
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