03134naa a2200469 a 450000100080000000500110000800800410001902400510006010000250011124501270013626000090026350001660027252015880043865000140202665000270204065000210206765000110208865000100209965000260210965300210213565300380215665300150219465300240220965300230223365300230225665300160227970000200229570000200231570000270233570000240236270000250238670000200241170000180243170000200244970000190246970000200248870000210250870000200252970000230254970000200257277300720259221540562023-05-29       2023    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1017/S000748532200061X2DOI1 aFONSECA, F. C. de A.  aIn vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer.h[electronic resource]  c2023  aNa publicação: Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessuti; Carolina Viana Morgante; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.  aThe sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields.  aSugarcane  aBacillus Thuringiensis  aCana de Açúcar  aInseto  aPraga  aSaccharum Officinarum  aAminopeptidase N  aBroca gigante da cana-de-açúcar  aCry toxins  aModelagem molecular  aMolecular modeling  aTechin licus licus  aToxinas Cry1 aANTONINO, J. D.1 aMOURA, S. M. de1 aRODRIGUES-SILVA, P. L.1 aMACEDO, L. L. P. de1 aGOMES JÚNIOR, J. E.1 aLOURENCO, I. T.1 aLUCENA, W. A.1 aMORGANTE, C. V.1 aRIBEIRO, T. P.1 aMONNERAT, R. G.1 aRODRIGUES, M. A.1 aCUCCOVIA, I. M.1 aSILVA, M. C. M. da1 aSA, M. F. G. de  tBulletin of Entomological Researchgv. 113, n. 3, p. 335-346, 2023.