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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
25/08/2022 |
Data da última atualização: |
05/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HUNGRIA, M.; BARBOSA, J. Z.; RONDINA, A. B. L.; NOGUEIRA, M. A. |
Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; IF SUDESTE MG; CNPQ; MARCO ANTONIO NOGUEIRA, CNPSO. |
Título: |
Improving maize sustainability with partial replacement of N fertilizers by inoculation with Azospirillum brasilense. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Agronomy Journal, v. 114, n. 5, p. 2969-2980, 2022. |
Páginas: |
12 p. |
DOI: |
10.1002/agj2.21150 |
Idioma: |
Inglês |
Thesagro: |
Fertilizante Nitrogenado; Inoculação; Milho. |
Thesaurus Nal: |
Corn; Inoculation methods; Nitrogen fertilizers. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 00741naa a2200241 a 4500 001 2145740 005 2023-01-05 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1002/agj2.21150$2DOI 100 1 $aHUNGRIA, M. 245 $aImproving maize sustainability with partial replacement of N fertilizers by inoculation with Azospirillum brasilense.$h[electronic resource] 260 $c2022 300 $a12 p. 650 $aCorn 650 $aInoculation methods 650 $aNitrogen fertilizers 650 $aFertilizante Nitrogenado 650 $aInoculação 650 $aMilho 700 1 $aBARBOSA, J. Z. 700 1 $aRONDINA, A. B. L. 700 1 $aNOGUEIRA, M. A. 773 $tAgronomy Journal$gv. 114, n. 5, p. 2969-2980, 2022.
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
19/09/2017 |
Data da última atualização: |
29/12/2017 |
Autoria: |
MASCARENHAS, M. B.; PEIXOTO, P. V.; RAMADINHA, R. R.; ARMIEN, A. G.; COSTA, S. Z.; MIRANDA, I. C.; NOGUEIRA, V. A.; FRANÇA, T. N. |
Afiliação: |
Mariana B. Mascarenhas, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Paulo V. Peixoto, Departamento de Nutrição e Pastagem/Instituto de Zootecnia/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Regina R. Ramadinha, Departamento de Medicina e Cirurgia Veterinária/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Anibal G. Armien, Veterinary Diagnostic Laboratory/Department of Veterinary Population Medicine/College of Veterinary Medicine/University of Minnesota; Samay Z. Costa, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Ileana C. Miranda, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Vivian A. Nogueira, Departamento de Epidemiologia e Saúde Pública/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Ticiana N. França, Departamento de Epidemiologia e Saúde Pública/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ. |
Título: |
Immunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazil |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 613-620, jun. 2017. |
Idioma: |
Inglês |
Notas: |
Título em português: Estudos imuno-histoquímico, lectino-histoquímico e ultraestrutural do Tumor Venéreo Transmissível Canino no Brasil. |
Conteúdo: |
Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin. MenosCanine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed s... Mostrar Tudo |
Palavras-Chave: |
Canine transmissible venereal tumor; CTVT; Lectin histochemistry; Lectino-histoquímica; Tumor venéreo transmissível canino (TVTC); Ultrastructural. |
Thesagro: |
Cão; Doença; Microscopia eletrônica. |
Thesaurus NAL: |
Animal pathology; Dogs; Immunohistochemistry. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163939/1/Immunohistochemical-lectin-histochemical.pdf
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Marc: |
LEADER 03079naa a2200361 a 4500 001 2075805 005 2017-12-29 008 2017 bl uuuu u00u1 u #d 100 1 $aMASCARENHAS, M. B. 245 $aImmunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazil$h[electronic resource] 260 $c2017 500 $aTítulo em português: Estudos imuno-histoquímico, lectino-histoquímico e ultraestrutural do Tumor Venéreo Transmissível Canino no Brasil. 520 $aCanine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin. 650 $aAnimal pathology 650 $aDogs 650 $aImmunohistochemistry 650 $aCão 650 $aDoença 650 $aMicroscopia eletrônica 653 $aCanine transmissible venereal tumor 653 $aCTVT 653 $aLectin histochemistry 653 $aLectino-histoquímica 653 $aTumor venéreo transmissível canino (TVTC) 653 $aUltrastructural 700 1 $aPEIXOTO, P. V. 700 1 $aRAMADINHA, R. R. 700 1 $aARMIEN, A. G. 700 1 $aCOSTA, S. Z. 700 1 $aMIRANDA, I. C. 700 1 $aNOGUEIRA, V. A. 700 1 $aFRANÇA, T. N. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 613-620, jun. 2017.
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