03079naa a2200361 a 450000100080000000500110000800800410001910000230006024501460008326000090022950001470023852018360038565000210222165000090224265000250225165000090227665000120228565000280229765300400232565300090236565300260237465300260240065300480242665300200247470000190249470000210251370000180253470000170255270000190256970000200258870000190260877300900262720758052017-12-29       2017    bl uuuu     u00u1 u    #d1 aMASCARENHAS, M. B.  aImmunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazilh[electronic resource]  c2017  aTítulo em português: Estudos imuno-histoquímico, lectino-histoquímico e ultraestrutural do Tumor Venéreo Transmissível Canino no Brasil.  aCanine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin.  aAnimal pathology  aDogs  aImmunohistochemistry  aCão  aDoença  aMicroscopia eletrônica  aCanine transmissible venereal tumor  aCTVT  aLectin histochemistry  aLectino-histoquímica  aTumor venéreo transmissível canino (TVTC)  aUltrastructural1 aPEIXOTO, P. V.1 aRAMADINHA, R. R.1 aARMIEN, A. G.1 aCOSTA, S. Z.1 aMIRANDA, I. C.1 aNOGUEIRA, V. A.1 aFRANÇA, T. N.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 37, n. 6, p. 613-620, jun. 2017.