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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/11/2010 |
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Data da última atualização: |
05/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PASQUAL, M.; PIO, L. A. S; SILVA, S. de O. e; DAVIDE, L. C; SEREJO, J. A. dos S.; ROCHA, H. S. |
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Afiliação: |
MOACIR PASQUAL, UFLA; LEILA APARECIDA SALLES PIO, UFLA; SEBASTIÃO DE OLIVEIRA E SILVA; LISETE CHAMMA DAVIDE, UFLA; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; HERMINIO SOUZA ROCHA, CNPMF. |
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Título: |
Chromosome doubling in musa acuminata colla. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM. |
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Volume: |
v.2 |
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Páginas: |
p. 757. |
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Idioma: |
Inglês |
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Notas: |
PDF. S18.201. |
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Conteúdo: |
The aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible. MenosThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 ... Mostrar Tudo |
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Palavras-Chave: |
Cultivares. |
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Thesagro: |
Banana. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02437naa a2200241 a 4500
001 1866599
005 2011-04-05
008 2010 bl uuuu u00u1 u #d
100 1 $aPASQUAL, M.
245 $aChromosome doubling in musa acuminata colla.
260 $c2010
300 $ap. 757. v.2
490 $vv.2
500 $aPDF. S18.201.
520 $aThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible.
650 $aBanana
653 $aCultivares
700 1 $aPIO, L. A. S
700 1 $aSILVA, S. de O. e
700 1 $aDAVIDE, L. C
700 1 $aSEREJO, J. A. dos S.
700 1 $aROCHA, H. S.
773 $tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
04/11/2015 |
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Data da última atualização: |
02/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AYRES, G. B. da S.; SOLARI, F. L.; TAQUITA, J.; ALMEIDA, C. de S. T. de; GULIAS GOMES, C. C.; JUCHEM, S. de O.; BLOCH JUNIOR, C. |
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Afiliação: |
Gabriela Berdet da Silva Ayres, UNIPAMPA; FABIANO LOPES SOLARI, CPPSUL; JORGE ALEX TAQUITA MELO, CENARGEN; Clarice de Souza Tavares de Almeida, UNIPAMPA; CLAUDIA CRISTINA GULIAS GOMES, CPPSUL; SERGIO DE OLIVEIRA JUCHEM, CPPSUL; CARLOS BLOCH JUNIOR, CENARGEN. |
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Título: |
Efeito do tempo de eluição da fase sólida sobre a intensidade do sinal das lactonas macrocíclicas extraídas em plasma bovino. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 5., 2015, Bagé. Resumos dos trabalhos... Bagé: Embrapa Pecuária Sul, 2015. |
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Páginas: |
p. 38. |
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Idioma: |
Português |
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Notas: |
Editora técnica Claudia Cristina Gulias Gomes. |
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Conteúdo: |
A quantidade máxima tolerável de lactonas macrocíclicas (LM) na carne para consumo humano é de 10 μg/kg de tecido muscular segundo a instrução normativa nº 11 (MAPA/SDA/PNCRB, 2014). |
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Palavras-Chave: |
Tandem. |
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Thesagro: |
Análise química; Espectrometria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132357/1/Ayres-et-al.pdf
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Marc: |
LEADER 01073nam a2200241 a 4500
001 2027844
005 2016-03-02
008 2015 bl uuuu u00u1 u #d
100 1 $aAYRES, G. B. da S.
245 $aEfeito do tempo de eluição da fase sólida sobre a intensidade do sinal das lactonas macrocíclicas extraídas em plasma bovino.$h[electronic resource]
260 $aIn: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 5., 2015, Bagé. Resumos dos trabalhos... Bagé: Embrapa Pecuária Sul$c2015
300 $ap. 38.
500 $aEditora técnica Claudia Cristina Gulias Gomes.
520 $aA quantidade máxima tolerável de lactonas macrocíclicas (LM) na carne para consumo humano é de 10 μg/kg de tecido muscular segundo a instrução normativa nº 11 (MAPA/SDA/PNCRB, 2014).
650 $aAnálise química
650 $aEspectrometria
653 $aTandem
700 1 $aSOLARI, F. L.
700 1 $aTAQUITA, J.
700 1 $aALMEIDA, C. de S. T. de
700 1 $aGULIAS GOMES, C. C.
700 1 $aJUCHEM, S. de O.
700 1 $aBLOCH JUNIOR, C.
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Embrapa Pecuária Sul (CPPSUL) |
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