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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MARTINS, G. R.; MARINHO, R. C.; BEZERRA-JUNIOR, R. Q.; CÂMARA, L. M. C.; ALBUQUERQUE-PINTO, L. C.; TEIXEIRA, M. F. S. |
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Afiliação: |
Gabrielle R. Martins, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rebeca C. Marinho, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rosivaldo Q. Bezerra-Junior, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Lilia M. C. Câmara, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Luiz C. Albuquerque-Pinto, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Maria F. S. Teixeira, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará. |
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Título: |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 643-649, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino. |
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Conteúdo: |
Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood. |
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Palavras-Chave: |
Células tronco; Citometria de fluxo; Diferenciação em linhagenm; Lineage differentiation; MSC; Stem cell. |
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Thesaurus Nal: |
Flow cytometry; Goats. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163981/1/Isolation-culture-and-characterization-1.pdf
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Marc: |
LEADER 02331naa a2200289 a 4500
001 2075898
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMARTINS, G. R.
245 $aIsolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino.
520 $aMesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.
650 $aFlow cytometry
650 $aGoats
653 $aCélulas tronco
653 $aCitometria de fluxo
653 $aDiferenciação em linhagenm
653 $aLineage differentiation
653 $aMSC
653 $aStem cell
700 1 $aMARINHO, R. C.
700 1 $aBEZERRA-JUNIOR, R. Q.
700 1 $aCÂMARA, L. M. C.
700 1 $aALBUQUERQUE-PINTO, L. C.
700 1 $aTEIXEIRA, M. F. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 643-649, jun. 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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| 5. |  | MARTINS, G. R.; MARINHO, R. C.; BEZERRA-JUNIOR, R. Q.; CÂMARA, L. M. C.; ALBUQUERQUE-PINTO, L. C.; TEIXEIRA, M. F. S. Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 643-649, jun. 2017. Título em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino.| Biblioteca(s): Embrapa Unidades Centrais. |
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