02331naa a2200289 a 450000100080000000500110000800800410001910000190006024501370007926000090021650001650022552012860039065000190167665000100169565300200170565300240172565300330174965300280178265300080181065300140181870000190183270000260185170000220187770000290189970000230192877300900195120758982017-12-29       2017    bl uuuu     u00u1 u    #d1 aMARTINS, G. R.  aIsolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood.h[electronic resource]  c2017  aTítulo em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino.  aMesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.  aFlow cytometry  aGoats  aCélulas tronco  aCitometria de fluxo  aDiferenciação em linhagenm  aLineage differentiation  aMSC  aStem cell1 aMARINHO, R. C.1 aBEZERRA-JUNIOR, R. Q.1 aCÂMARA, L. M. C.1 aALBUQUERQUE-PINTO, L. C.1 aTEIXEIRA, M. F. S.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 37, n. 6, p. 643-649, jun. 2017.