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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
29/01/2008 |
Data da última atualização: |
24/04/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
PEREIRA, M. M.; VIANA, S. W.; BOITÉ, M. C.; CARVALHO, B. C.; BATISTA, R. I. T. P.; MOTA, G. B.; SERAPIÃO, R. V.; POLISSENI, J.; VIANA, J. H. M.; CAMARGO, L. S. A. |
Afiliação: |
Michele Munk Pereira, CES-JF; Sabine Wohlres Viana, UFJF; Mariana Cortes Boité, UFF; Bruno Campos de Carvalho, Epamig; Ribrio Ivan Tavares Pereira Batista, CES-JF; Gustavo Bruno Mota, UFV; Raquel Varela Serapião, UENF; Juliana Polisseni, UFJF; João Henrique Moreira Viana, Embrapa Gado de Leite; Luiz Sérgio Almeida Camargo, Embrapa Gado de Leite. |
Título: |
Influência da qualidade morfológica dos complexos oócito-cumulus na maturação nuclear e nas taxas de blastocistos bovinos. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: SEMANA DE BIOLOGIA, 30., 2007, Juiz de Fora. O mundo se tornou pequeno para você mas você não é grande para o mundo: anais. Juiz de Fora: DACBIO/UFJF, 2007. |
Páginas: |
p. 73-78. |
Idioma: |
Português |
Palavras-Chave: |
COCs; embriões bovinos; morfologia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/595407/1/Influencia-da-qualidade-morfologica-dos-complexos-oocito-cumulus.pdf
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Marc: |
LEADER 00893nam a2200253 a 4500 001 1595407 005 2024-04-24 008 2007 bl uuuu u00u1 u #d 100 1 $aPEREIRA, M. M. 245 $aInfluência da qualidade morfológica dos complexos oócito-cumulus na maturação nuclear e nas taxas de blastocistos bovinos.$h[electronic resource] 260 $aIn: SEMANA DE BIOLOGIA, 30., 2007, Juiz de Fora. O mundo se tornou pequeno para você mas você não é grande para o mundo: anais. Juiz de Fora: DACBIO/UFJF$c2007 300 $ap. 73-78. 653 $aCOCs 653 $aembriões bovinos 653 $amorfologia 700 1 $aVIANA, S. W. 700 1 $aBOITÉ, M. C. 700 1 $aCARVALHO, B. C. 700 1 $aBATISTA, R. I. T. P. 700 1 $aMOTA, G. B. 700 1 $aSERAPIÃO, R. V. 700 1 $aPOLISSENI, J. 700 1 $aVIANA, J. H. M. 700 1 $aCAMARGO, L. S. A.
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Embrapa Gado de Leite (CNPGL) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
13/03/2014 |
Data da última atualização: |
19/10/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
FARIA, M. T. de; CURY-BOAVENTURA, M. F.; LOPES, L. R.; SILVA, J. R. M. C. da. |
Afiliação: |
MARCOS TUCUNDUVA DE FARIA, CPATU; Maria Fernanda Cury-Boaventura, USP; Lucia Rossetti Lopes, USP; José Roberto Machado Cunha da Silva, USP. |
Título: |
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Fish Physiology and Biochemistry, v. 40, n. 2, p. 445-455, Apr. 2014. |
DOI: |
10.1007/s10695-013-9856-9 |
Idioma: |
Inglês |
Conteúdo: |
Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. MenosProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the r... Mostrar Tudo |
Palavras-Chave: |
Citometria de fluxo; Espécies reativas de oxigênio; Explosão respiratória; Mitocôndrias. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02340naa a2200217 a 4500 001 1982284 005 2022-10-19 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s10695-013-9856-9$2DOI 100 1 $aFARIA, M. T. de 245 $aGeneration of reactive oxygen species by leukocytes of Prochilodus lineatus.$h[electronic resource] 260 $c2014 520 $aProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. 653 $aCitometria de fluxo 653 $aEspécies reativas de oxigênio 653 $aExplosão respiratória 653 $aMitocôndrias 700 1 $aCURY-BOAVENTURA, M. F. 700 1 $aLOPES, L. R. 700 1 $aSILVA, J. R. M. C. da 773 $tFish Physiology and Biochemistry$gv. 40, n. 2, p. 445-455, Apr. 2014.
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