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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
18/12/2013 |
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Data da última atualização: |
28/02/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, I. S. de; SANTOS, S. N.; ROSA, L. H.; PARMA, M. M.; SILVA, L. J.; QUEIROZ, S. C. do N. de; PELLIZARI, V. H. |
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Afiliação: |
ITAMAR SOARES DE MELO, CNPMA; SUIKINAI NOBRE SANTOS, ESALQ-USP; LUIZ HENRIQUE ROSA, ICB-UFMG; MARCIA MARIA PARMA, CNPMA; LEONARDO J SILVA; SONIA CLAUDIA DO NASCIMENTO DE QUEIROZ, CNPMA; VIVAN HELENA PELLIZARI, IO-USP. |
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Título: |
Isolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Extremophiles, Tokyo, v. 18, n. 1, p. 15-23, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The Antarctic endophytic fungus (strain ITA1-CCMA 952) was isolated from the moss Schistidium antarctici found in Admiralty Bay, King George Island, Antarctica. Strain ITA1-CCMA 952 was assigned to the specie Mortierella alpina by phylogenetic analysis based on 18S rRNA gene sequences. This strain produces high levels of polyunsaturated fatty acids (PUFAs), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3 % of the total fatty acid content. Fungal extracts demonstrated strong antioxidant activity with the EC50 value of 48.7 lg mL-1 and also a strong antibacterial activity, mainly against the following bacteria: Escherichia coli, with a MIC of 26.9 lg mL-1 and Pseudomonas aeruginosa and Enterococcus faecalis, both with a MIC of 107 lg mL-1. A GC-MS analysis of the chloroform fraction obtained from the crude extract revealed the presence of potential antimicrobials (Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)) as the major compounds. Therefore, the M. alpina strain ITA1-CCMA 952 is a promising fungus for the biotechnological production of antibiotics, antioxidant substances and PUFAs. This study highlights the need for more research in extreme environments, such as Antarctica. |
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Palavras-Chave: |
Antibacterial activity; PUFAs; Pyrrolopyrazine alkaloids; Schistidium antarctici. |
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Thesagro: |
Bactéria; Fungo. |
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Thesaurus Nal: |
Antarctica; Antibacterial properties; Entomopathogenic fungi; Mortierella. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02258naa a2200313 a 4500
001 1974294
005 2014-02-28
008 2014 bl uuuu u00u1 u #d
100 1 $aMELO, I. S. de
245 $aIsolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici.$h[electronic resource]
260 $c2014
520 $aThe Antarctic endophytic fungus (strain ITA1-CCMA 952) was isolated from the moss Schistidium antarctici found in Admiralty Bay, King George Island, Antarctica. Strain ITA1-CCMA 952 was assigned to the specie Mortierella alpina by phylogenetic analysis based on 18S rRNA gene sequences. This strain produces high levels of polyunsaturated fatty acids (PUFAs), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3 % of the total fatty acid content. Fungal extracts demonstrated strong antioxidant activity with the EC50 value of 48.7 lg mL-1 and also a strong antibacterial activity, mainly against the following bacteria: Escherichia coli, with a MIC of 26.9 lg mL-1 and Pseudomonas aeruginosa and Enterococcus faecalis, both with a MIC of 107 lg mL-1. A GC-MS analysis of the chloroform fraction obtained from the crude extract revealed the presence of potential antimicrobials (Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)) as the major compounds. Therefore, the M. alpina strain ITA1-CCMA 952 is a promising fungus for the biotechnological production of antibiotics, antioxidant substances and PUFAs. This study highlights the need for more research in extreme environments, such as Antarctica.
650 $aAntarctica
650 $aAntibacterial properties
650 $aEntomopathogenic fungi
650 $aMortierella
650 $aBactéria
650 $aFungo
653 $aAntibacterial activity
653 $aPUFAs
653 $aPyrrolopyrazine alkaloids
653 $aSchistidium antarctici
700 1 $aSANTOS, S. N.
700 1 $aROSA, L. H.
700 1 $aPARMA, M. M.
700 1 $aSILVA, L. J.
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aPELLIZARI, V. H.
773 $tExtremophiles, Tokyo$gv. 18, n. 1, p. 15-23, 2014.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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