02258naa a2200313 a 450000100080000000500110000800800410001910000190006024501540007926000090023352013250024265000150156765000290158265000270161165000160163865000140165465000100166865300270167865300100170565300300171565300270174570000180177270000160179070000170180670000170182370000280184070000210186877300550188919742942014-02-28       2014    bl uuuu     u00u1 u    #d1 aMELO, I. S. de  aIsolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici.h[electronic resource]  c2014  aThe Antarctic endophytic fungus (strain ITA1-CCMA 952) was isolated from the moss Schistidium antarctici found in Admiralty Bay, King George Island, Antarctica. Strain ITA1-CCMA 952 was assigned to the specie Mortierella alpina by phylogenetic analysis based on 18S rRNA gene sequences. This strain produces high levels of polyunsaturated fatty acids (PUFAs), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3 % of the total fatty acid content. Fungal extracts demonstrated strong antioxidant activity with the EC50 value of 48.7 lg mL-1 and also a strong antibacterial activity, mainly against the following bacteria: Escherichia coli, with a MIC of 26.9 lg mL-1 and Pseudomonas aeruginosa and Enterococcus faecalis, both with a MIC of 107 lg mL-1. A GC-MS analysis of the chloroform fraction obtained from the crude extract revealed the presence of potential antimicrobials (Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)) as the major compounds. Therefore, the M. alpina strain ITA1-CCMA 952 is a promising fungus for the biotechnological production of antibiotics, antioxidant substances and PUFAs. This study highlights the need for more research in extreme environments, such as Antarctica.  aAntarctica  aAntibacterial properties  aEntomopathogenic fungi  aMortierella  aBactéria  aFungo  aAntibacterial activity  aPUFAs  aPyrrolopyrazine alkaloids  aSchistidium antarctici1 aSANTOS, S. N.1 aROSA, L. H.1 aPARMA, M. M.1 aSILVA, L. J.1 aQUEIROZ, S. C. do N. de1 aPELLIZARI, V. H.  tExtremophiles, Tokyogv. 18, n. 1, p. 15-23, 2014.