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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
Data corrente: |
25/05/2015 |
Data da última atualização: |
05/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z. |
Afiliação: |
Carolina Cerqueira Sarmento Olivares, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; André Luís Rios Rodrigues, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil; Felipe Zandonadi Brandão, Faculty of Veterinary Medicine, Fluminense Federal University - Niterói, RJ, Brazil. |
Título: |
Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 127, p. 44-49, June, 2015. |
DOI: |
doi:10.1016/j.smallrumres.2015.04.009 |
Idioma: |
Inglês Português |
Conteúdo: |
Abstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment MenosAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin t... Mostrar Tudo |
Palavras-Chave: |
Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection. |
Thesagro: |
Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen. |
Thesaurus Nal: |
goats; reproduction; spermatozoa. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02549naa a2200337 a 4500 001 2016097 005 2018-02-05 008 2015 bl uuuu u00u1 u #d 024 7 $adoi:10.1016/j.smallrumres.2015.04.009$2DOI 100 1 $aOLIVARES, C. C. S. 245 $aComparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.$h[electronic resource] 260 $c2015 520 $aAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment 650 $agoats 650 $areproduction 650 $aspermatozoa 650 $aCaprino 650 $aEspermatozóide 650 $aReprodução animal 650 $aSeleção 650 $aSêmen 653 $aCapacitation 653 $aSodium nitroprusside 653 $aSperm preparation 653 $aSperm selection 700 1 $aFONSECA, J. F. da 700 1 $aCAMARGO, L. S. de A. 700 1 $aFABJAN, J. M. G. de S. 700 1 $aRODRIGUES, A. L. R. 700 1 $aBRANDÃO, F. Z. 773 $tSmall Ruminant Research, Amsterdam$gv. 127, p. 44-49, June, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
03/01/2020 |
Data da última atualização: |
03/01/2020 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FIGUEIREDO, M. C.; SILVA, G. R. da; UCHOA, F. P.; SILVA, R. M. de L.; RUDNICK, V. A. de S.; VIEIRA JUNIOR, J. R. |
Afiliação: |
Matheus Cunha Figueiredo; Gleicele Ribeiro da Silva; Francisco Paiva Uchoa; Rebeca Mona de Lima Silva; Vaneide Araújo de Sousa Rudnick; JOSE ROBERTO VIEIRA JUNIOR, CPAF-RO. |
Título: |
Resistência dos clones Robusta Amazônico a nematoide-das-galhas. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 10.; ENCONTRO DE PÓS-GRADUAÇÃO DA EMBRAPA RONDÔNIA, 5., 2019, Porto Velho, RO. Anais... Porto Velho, RO: Embrapa Rondônia, 2019. |
Páginas: |
p. 38. |
Idioma: |
Português |
Conteúdo: |
Este trabalho objetivou caracterizar a resposta de resistência à M. incognita em 12 clones de Coffea canephora e das variedades botânicas Conilon, Robusta e seus híbridos. |
Palavras-Chave: |
Coffe canephora. |
Thesagro: |
Meloidogyne Incognita. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208068/1/Resumo-38.pdf
|
Marc: |
LEADER 00928nam a2200205 a 4500 001 2118038 005 2020-01-03 008 2019 bl uuuu u00u1 u #d 100 1 $aFIGUEIREDO, M. C. 245 $aResistência dos clones Robusta Amazônico a nematoide-das-galhas.$h[electronic resource] 260 $aIn: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 10.; ENCONTRO DE PÓS-GRADUAÇÃO DA EMBRAPA RONDÔNIA, 5., 2019, Porto Velho, RO. Anais... Porto Velho, RO: Embrapa Rondônia$c2019 300 $ap. 38. 520 $aEste trabalho objetivou caracterizar a resposta de resistência à M. incognita em 12 clones de Coffea canephora e das variedades botânicas Conilon, Robusta e seus híbridos. 650 $aMeloidogyne Incognita 653 $aCoffe canephora 700 1 $aSILVA, G. R. da 700 1 $aUCHOA, F. P. 700 1 $aSILVA, R. M. de L. 700 1 $aRUDNICK, V. A. de S. 700 1 $aVIEIRA JUNIOR, J. R.
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