02549naa a2200337 a 450000100080000000500110000800800410001902400470006010000230010724501560013026000090028652015200029565000100181565000170182565000160184265000120185865000200187065000240189065000140191465000110192865300170193965300250195665300220198165300200200370000220202370000250204570000270207070000240209770000200212177300700214120160972018-02-05       2015    bl uuuu     u00u1 u    #d7 adoi:10.1016/j.smallrumres.2015.04.0092DOI1 aOLIVARES, C. C. S.  aComparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.h[electronic resource]  c2015  aAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P &lt 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P &lt 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P &lt 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P &lt 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment  agoats  areproduction  aspermatozoa  aCaprino  aEspermatozóide  aReprodução animal  aSeleção  aSêmen  aCapacitation  aSodium nitroprusside  aSperm preparation  aSperm selection1 aFONSECA, J. F. da1 aCAMARGO, L. S. de A.1 aFABJAN, J. M. G. de S.1 aRODRIGUES, A. L. R.1 aBRANDÃO, F. Z.  tSmall Ruminant Research, Amsterdamgv. 127, p. 44-49, June, 2015.