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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/02/2012 |
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Data da última atualização: |
08/07/2016 |
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Autoria: |
MOURA, L. C. O.; SILVA, M. C. da; SNOECK, P. P. da N. |
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Título: |
Diferentes soluções de teste hiposmótico para sêmen ovino. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Medicina Veterinária, Rio de Janeiro, v. 32, n. 3, p. 146-150, jul./set., 2010. |
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Idioma: |
Português |
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Conteúdo: |
O teste hiposmótico (HO) vem sendo usado para avaliar a integridade funcional da membrana espermática de várias espécies de mamíferos domésticos e baseia-se na troca de fluido do meio intracelular com o meio extracelular até que haja um equilíbrio. O objetivo deste estudo foi comparar duas diferentes soluções de teste HO para sêmen ovino fresco e resfriado, utilizando as soluções de frutose-citrato de sódio e sacarose, ambas a 100mOsmol/L. Foram utilizadas 8 amostras de sêmen fresco e 128 amostras de sêmen resfriado. Para cada amostra de sêmen foram retiradas duas alíquotas de 25μL. Uma alíquota foi adicionada em um eppendorf 4 contendo 200μL de solução de frutose-citrato de sódio e a outra em eppendorf contendo 200μL de solução de sacarose. As duas amostras foram incubadas em banho-maria a 37o C, por 30 minutos e posteriormente fixadas com 300μL de formol salina tamponada. A leitura das formas reativas foi realizada em microscopia de contraste de fase, com objetiva de imersão de 100X. O percentual de formas reativas, com membrana funcionalmente íntegra, foi avaliado após contagem dos espermatozóides com dobramento e/ou enrolamento de cauda e também após subtração das patologias de cauda encontradas antes do teste. Para o sêmen fresco, as soluções de frutose-citrato de sódio e sacarose foram semelhantes (P>0,05), na identificação do percentual de formas reativas, quando a fórmula de cálculo foi aplicada. Quando a fórmula não foi utilizada, verificou-se que a frutose-citrato de sódio foi superior a sacarose (P<0,05). As amostras de sêmen resfriado, antes e após submeter os dados à fórmula de cálculo reagiram diferentemente, dependendo da solução HO utilizada, sendo a frutose-citrato de sódio (P<0,05) a melhor solução para a realização deste teste HO em sêmen resfriado de ovino.
[Different solutions of hypoosmotic test for ram semen].
Abstract - The hypoosmotic test (HOS) has even used to evaluate functional integrity of the sperm membrane of many domestic mammals species and is based on the fluids exchange from the intracellular with the extracellular medium until a balance is accomplished. The aim of this study was to compare two different solutions of HOS test for fresh and cooled ram semen, using the fructose-sodium citrate and sucrose solutions, both with 100 mOsmol/L. Eight fresh semen samples and 128 cooled semen samples were evaluated. For each semen sample, two aliquots of 25 μL were taken. One aliquot was added in eppendorf, containing 200 μL fructose-sodium citrate and another was added to 200 mL sucrose solution. The two samples were incubated for thirty minutes at 37o C in water bath and then fixed in 300 μL buffered formalin saline solution. The reactive form was observed in a phase-contrast microscope with oil immersion. The percentage of reactive forms, with functionally membrane, was evaluated after the counting of the coiled/bent tails and either after subtracted of the abnormal tails found before the test. To the fresh semen, the fructose-citrate sodium and the sucrose solution were similar (P>0.05), in the recognition of the reactive forms perceptual, when the count formula was applied. When the count formula was not use, was verified that the fructose-citrate sodium were better than the sucrose (P<0.05). The fructose-citrate sodium solution was better to identify maximal swelling spermatozoa than sucrose solution after HOS test in cooled ram semen (P<0.05). MenosO teste hiposmótico (HO) vem sendo usado para avaliar a integridade funcional da membrana espermática de várias espécies de mamíferos domésticos e baseia-se na troca de fluido do meio intracelular com o meio extracelular até que haja um equilíbrio. O objetivo deste estudo foi comparar duas diferentes soluções de teste HO para sêmen ovino fresco e resfriado, utilizando as soluções de frutose-citrato de sódio e sacarose, ambas a 100mOsmol/L. Foram utilizadas 8 amostras de sêmen fresco e 128 amostras de sêmen resfriado. Para cada amostra de sêmen foram retiradas duas alíquotas de 25μL. Uma alíquota foi adicionada em um eppendorf 4 contendo 200μL de solução de frutose-citrato de sódio e a outra em eppendorf contendo 200μL de solução de sacarose. As duas amostras foram incubadas em banho-maria a 37o C, por 30 minutos e posteriormente fixadas com 300μL de formol salina tamponada. A leitura das formas reativas foi realizada em microscopia de contraste de fase, com objetiva de imersão de 100X. O percentual de formas reativas, com membrana funcionalmente íntegra, foi avaliado após contagem dos espermatozóides com dobramento e/ou enrolamento de cauda e também após subtração das patologias de cauda encontradas antes do teste. Para o sêmen fresco, as soluções de frutose-citrato de sódio e sacarose foram semelhantes (P>0,05), na identificação do percentual de formas reativas, quando a fórmula de cálculo foi aplicada. Quando a fórmula não foi utilizada, verificou-se q... Mostrar Tudo |
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Palavras-Chave: |
Frutose-citrato de sódio; Pressão osmótica; Teste hiposmótico. |
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Thesagro: |
Carneiro; Espermatozóide; Ovino; Reprodução animal; Sacarose; Sêmen; Solução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04326naa a2200265 a 4500
001 1914716
005 2016-07-08
008 2010 bl uuuu u00u1 u #d
100 1 $aMOURA, L. C. O.
245 $aDiferentes soluções de teste hiposmótico para sêmen ovino.$h[electronic resource]
260 $c2010
520 $aO teste hiposmótico (HO) vem sendo usado para avaliar a integridade funcional da membrana espermática de várias espécies de mamíferos domésticos e baseia-se na troca de fluido do meio intracelular com o meio extracelular até que haja um equilíbrio. O objetivo deste estudo foi comparar duas diferentes soluções de teste HO para sêmen ovino fresco e resfriado, utilizando as soluções de frutose-citrato de sódio e sacarose, ambas a 100mOsmol/L. Foram utilizadas 8 amostras de sêmen fresco e 128 amostras de sêmen resfriado. Para cada amostra de sêmen foram retiradas duas alíquotas de 25μL. Uma alíquota foi adicionada em um eppendorf 4 contendo 200μL de solução de frutose-citrato de sódio e a outra em eppendorf contendo 200μL de solução de sacarose. As duas amostras foram incubadas em banho-maria a 37o C, por 30 minutos e posteriormente fixadas com 300μL de formol salina tamponada. A leitura das formas reativas foi realizada em microscopia de contraste de fase, com objetiva de imersão de 100X. O percentual de formas reativas, com membrana funcionalmente íntegra, foi avaliado após contagem dos espermatozóides com dobramento e/ou enrolamento de cauda e também após subtração das patologias de cauda encontradas antes do teste. Para o sêmen fresco, as soluções de frutose-citrato de sódio e sacarose foram semelhantes (P>0,05), na identificação do percentual de formas reativas, quando a fórmula de cálculo foi aplicada. Quando a fórmula não foi utilizada, verificou-se que a frutose-citrato de sódio foi superior a sacarose (P<0,05). As amostras de sêmen resfriado, antes e após submeter os dados à fórmula de cálculo reagiram diferentemente, dependendo da solução HO utilizada, sendo a frutose-citrato de sódio (P<0,05) a melhor solução para a realização deste teste HO em sêmen resfriado de ovino. [Different solutions of hypoosmotic test for ram semen]. Abstract - The hypoosmotic test (HOS) has even used to evaluate functional integrity of the sperm membrane of many domestic mammals species and is based on the fluids exchange from the intracellular with the extracellular medium until a balance is accomplished. The aim of this study was to compare two different solutions of HOS test for fresh and cooled ram semen, using the fructose-sodium citrate and sucrose solutions, both with 100 mOsmol/L. Eight fresh semen samples and 128 cooled semen samples were evaluated. For each semen sample, two aliquots of 25 μL were taken. One aliquot was added in eppendorf, containing 200 μL fructose-sodium citrate and another was added to 200 mL sucrose solution. The two samples were incubated for thirty minutes at 37o C in water bath and then fixed in 300 μL buffered formalin saline solution. The reactive form was observed in a phase-contrast microscope with oil immersion. The percentage of reactive forms, with functionally membrane, was evaluated after the counting of the coiled/bent tails and either after subtracted of the abnormal tails found before the test. To the fresh semen, the fructose-citrate sodium and the sucrose solution were similar (P>0.05), in the recognition of the reactive forms perceptual, when the count formula was applied. When the count formula was not use, was verified that the fructose-citrate sodium were better than the sucrose (P<0.05). The fructose-citrate sodium solution was better to identify maximal swelling spermatozoa than sucrose solution after HOS test in cooled ram semen (P<0.05).
650 $aCarneiro
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSacarose
650 $aSêmen
650 $aSolução
653 $aFrutose-citrato de sódio
653 $aPressão osmótica
653 $aTeste hiposmótico
700 1 $aSILVA, M. C. da
700 1 $aSNOECK, P. P. da N.
773 $tRevista Brasileira de Medicina Veterinária, Rio de Janeiro$gv. 32, n. 3, p. 146-150, jul./set., 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/09/2008 |
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Data da última atualização: |
24/09/2008 |
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Autoria: |
KUPERMAN, R. G.; CHECKAI, R. T.; ROMBKE, J.; STEPHENSON. G. L.; SOUSA, J. P. |
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Título: |
Ecotoxicological assessment of contaminated land. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Over the past two decades, soil ecotoxicologists have made strides in utilizing the basic concepts
and advancements in soil zoology and ecology. They have applied the existing tools, and
developed new tools to investigate how chemical contamination can affect soil ecosystems,
including the degradation or destruction of soil habitats, diminishment of below-ground biodiversity,
and negative impacts on complex food webs and the associated above-ground biotic
communities. Soil ecotoxicologists at present are applying a suite of standard protocols, originally
developed for laboratory soil toxicity testing, and further enhancing both the approaches and
protocols for the assessment of contaminated lands. The current challenge is how to integrate
species-specific sensitivities, the effects of physical and chemical soil properties, contaminant
bioavailability and toxicity, uptake and bioconcentration, and spatial and temporal variations in
abundance and diversity of soil organisms, into coherent toxicological assessments of soil
ecosystems amid contaminated lands. This overview will attempt to address the question that
usually confronts an ecotoxicologist in any assessment of a soil ecosystem: ?Which terrestrial
toxicity tests constitute an appropriate test battery?? There is a plethora of methods that focus
on the assessment of soils via spiking, or amendment, with chemicals or other test substances.
However, only those methods that contain procedures for assessing contaminated site-specific
soils (or those methods that can easily be adapted for that purpose) are recommended for such
a test battery, and those will be discussed in this overview. Important considerations that will be
addressed include: 1) a need for a greatly improved understanding of the potential effects of
chemical contaminants on the structure and function of soil ecosystems; and 2) ready availability
of reliable appropriate soil toxicity test methods that have been developed explicitly to assess
the toxicity of contaminated site soils. International soil toxicity test methods that have been
standardized, or those that are in the process of being standardized, will also be reviewed. MenosOver the past two decades, soil ecotoxicologists have made strides in utilizing the basic concepts
and advancements in soil zoology and ecology. They have applied the existing tools, and
developed new tools to investigate how chemical contamination can affect soil ecosystems,
including the degradation or destruction of soil habitats, diminishment of below-ground biodiversity,
and negative impacts on complex food webs and the associated above-ground biotic
communities. Soil ecotoxicologists at present are applying a suite of standard protocols, originally
developed for laboratory soil toxicity testing, and further enhancing both the approaches and
protocols for the assessment of contaminated lands. The current challenge is how to integrate
species-specific sensitivities, the effects of physical and chemical soil properties, contaminant
bioavailability and toxicity, uptake and bioconcentration, and spatial and temporal variations in
abundance and diversity of soil organisms, into coherent toxicological assessments of soil
ecosystems amid contaminated lands. This overview will attempt to address the question that
usually confronts an ecotoxicologist in any assessment of a soil ecosystem: ?Which terrestrial
toxicity tests constitute an appropriate test battery?? There is a plethora of methods that focus
on the assessment of soils via spiking, or amendment, with chemicals or other test substances.
However, only those methods that contain procedures for assessing contaminated site... Mostrar Tudo |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02893naa a2200169 a 4500
001 1314868
005 2008-09-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKUPERMAN, R. G.
245 $aEcotoxicological assessment of contaminated land.
260 $c2008
520 $aOver the past two decades, soil ecotoxicologists have made strides in utilizing the basic concepts and advancements in soil zoology and ecology. They have applied the existing tools, and developed new tools to investigate how chemical contamination can affect soil ecosystems, including the degradation or destruction of soil habitats, diminishment of below-ground biodiversity, and negative impacts on complex food webs and the associated above-ground biotic communities. Soil ecotoxicologists at present are applying a suite of standard protocols, originally developed for laboratory soil toxicity testing, and further enhancing both the approaches and protocols for the assessment of contaminated lands. The current challenge is how to integrate species-specific sensitivities, the effects of physical and chemical soil properties, contaminant bioavailability and toxicity, uptake and bioconcentration, and spatial and temporal variations in abundance and diversity of soil organisms, into coherent toxicological assessments of soil ecosystems amid contaminated lands. This overview will attempt to address the question that usually confronts an ecotoxicologist in any assessment of a soil ecosystem: ?Which terrestrial toxicity tests constitute an appropriate test battery?? There is a plethora of methods that focus on the assessment of soils via spiking, or amendment, with chemicals or other test substances. However, only those methods that contain procedures for assessing contaminated site-specific soils (or those methods that can easily be adapted for that purpose) are recommended for such a test battery, and those will be discussed in this overview. Important considerations that will be addressed include: 1) a need for a greatly improved understanding of the potential effects of chemical contaminants on the structure and function of soil ecosystems; and 2) ready availability of reliable appropriate soil toxicity test methods that have been developed explicitly to assess the toxicity of contaminated site soils. International soil toxicity test methods that have been standardized, or those that are in the process of being standardized, will also be reviewed.
700 1 $aCHECKAI, R. T.
700 1 $aROMBKE, J.
700 1 $aSTEPHENSON. G. L.
700 1 $aSOUSA, J. P.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Download
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Embrapa Florestas (CNPF) |
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