| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pantanal. |
|
Data corrente: |
16/07/2010 |
|
Data da última atualização: |
16/07/2010 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PEDRINI, S. C. B.; ROSA, P. S.; MEDRI, I. M.; MOURAO, G. de M.; BAGAGLI, E.; LOPES, C. A. de M. |
|
Afiliação: |
SILVIA CRISTINA BARBOZA PEDRINI, INSTITUTO LAURO DE SOUZA LIMA; PATRÍCIA SAMMARCO ROSA, INSTITUTO LAURO DE SOUZA LIMA; ÍSIS MERI MEDRI; GUILHERME DE MIRANDA MOURAO, CPAP; EDUARDO BAGAGLI, UNESP; CARLOS ALBERTO DE MAGALHÃES LOPES, UNESP. |
|
Título: |
Search for Mycobacterium leprae in wild mammals. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Brazilian Journal of Infectious Diseases, v. 14, n.1, p. 47-53, 2010. |
|
Idioma: |
Português |
|
Conteúdo: |
Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the
experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed
samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state. MenosLeprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the
experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed
samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected f... Mostrar Tudo |
|
Palavras-Chave: |
Eco-epidemiology; Euphractus sexcinctus; Wild mammals. |
|
Thesagro: |
Dasypus Novemcinctus. |
|
Thesaurus Nal: |
Cabassous tatouay; Mycobacterium leprae. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02624naa a2200253 a 4500
001 1858047
005 2010-07-16
008 2010 bl --- 0-- u #d
100 1 $aPEDRINI, S. C. B.
245 $aSearch for Mycobacterium leprae in wild mammals.
260 $c2010
520 $aLeprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state.
650 $aCabassous tatouay
650 $aMycobacterium leprae
650 $aDasypus Novemcinctus
653 $aEco-epidemiology
653 $aEuphractus sexcinctus
653 $aWild mammals
700 1 $aROSA, P. S.
700 1 $aMEDRI, I. M.
700 1 $aMOURAO, G. de M.
700 1 $aBAGAGLI, E.
700 1 $aLOPES, C. A. de M.
773 $tBrazilian Journal of Infectious Diseases$gv. 14, n.1, p. 47-53, 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
09/03/2004 |
|
Data da última atualização: |
04/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FAGERIA, N. K.; BALIGAR, V. C. |
|
Afiliação: |
NAND KUMAR FAGERIA, CNPAF; V. C. BALIGAR, USDA. |
|
Título: |
Methodology for evaluation of lowland rice genotypes for nitrogen use efficiency. |
|
Ano de publicação: |
2003 |
|
Fonte/Imprenta: |
Journal of Plant Nutrition, v. 26, n. 6, p. 1315-1333, June 2003. |
|
DOI: |
https://doi.org/10.1081/PLN-120020373 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Rice is a staple food for more than 50% of the world's population. Based on land and water management practices, rice ecosystem is mainly divided into lowland, upland, and deep water or floating rice. However, major area and production at global level comes from lowland or flooded rice system. In rice growing regions nitrogen (N) is one of the most yield-limiting nutrients for rice production. Adaptation of cultivars or genotypes with high N use efficiency is a potential strategy in optimizing N requirements of crops, lowering the cost of production and reducing the environmental pollution. The objectives of this paper are to discuss rate and timing of N application, define N?use efficiency, discuss mechanisms involved for genotypic variation in N?use efficiency and present experimental evidence of genotypic variations in N-use efficiency in lowland rice. Evaluation methodology and criteria for screening N-use efficiency are also discussed. Significant variation in N use efficiency exists in lowland rice genotypes. Nitrogen use efficiency parameters (grain yield per unit of N uptake, grain yield per unit of N applied and recovery of applied N) are useful in differentiating lowland rice genotypes into efficient and non?efficient responders to applied N. Such an evaluation could assist in identification of elite genotypes that could be used in breeding program to produce cultivars with high N use efficiency and capable of producing high yields. |
|
Palavras-Chave: |
Nutrient use efficiency mechanisms; Screening for N efficiency. |
|
Thesagro: |
Arroz; Genótipo; Nitrogênio; Oryza Sativa. |
|
Thesaurus NAL: |
grain yield; rice. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02198naa a2200241 a 4500
001 1211938
005 2022-05-04
008 2003 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1081/PLN-120020373$2DOI
100 1 $aFAGERIA, N. K.
245 $aMethodology for evaluation of lowland rice genotypes for nitrogen use efficiency.$h[electronic resource]
260 $c2003
520 $aRice is a staple food for more than 50% of the world's population. Based on land and water management practices, rice ecosystem is mainly divided into lowland, upland, and deep water or floating rice. However, major area and production at global level comes from lowland or flooded rice system. In rice growing regions nitrogen (N) is one of the most yield-limiting nutrients for rice production. Adaptation of cultivars or genotypes with high N use efficiency is a potential strategy in optimizing N requirements of crops, lowering the cost of production and reducing the environmental pollution. The objectives of this paper are to discuss rate and timing of N application, define N?use efficiency, discuss mechanisms involved for genotypic variation in N?use efficiency and present experimental evidence of genotypic variations in N-use efficiency in lowland rice. Evaluation methodology and criteria for screening N-use efficiency are also discussed. Significant variation in N use efficiency exists in lowland rice genotypes. Nitrogen use efficiency parameters (grain yield per unit of N uptake, grain yield per unit of N applied and recovery of applied N) are useful in differentiating lowland rice genotypes into efficient and non?efficient responders to applied N. Such an evaluation could assist in identification of elite genotypes that could be used in breeding program to produce cultivars with high N use efficiency and capable of producing high yields.
650 $agrain yield
650 $arice
650 $aArroz
650 $aGenótipo
650 $aNitrogênio
650 $aOryza Sativa
653 $aNutrient use efficiency mechanisms
653 $aScreening for N efficiency
700 1 $aBALIGAR, V. C.
773 $tJournal of Plant Nutrition$gv. 26, n. 6, p. 1315-1333, June 2003.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
