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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
11/01/2019 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATTOS, B. B.; MONTEBIANCO, C.; ROMANEL, E.; SILVA, T. da F.; BERNABÉ, R. B.; SIMAS-TOSIN, F.; SOUZA, L. M.; SASSAKI, G. L.; VASLIN, M. F. S.; BARRETO-BERGTER, E. |
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Afiliação: |
BIANCA BRAZ MATTOS, CNPS; CAROLINE MONTEBIANCO, UFRJ; ELISSON ROMANEL, UFRJ/USP; TATIANE DA FRANCA SILVA, UFRJ/USP; RENATO BARROSO BERNABÉ, UFRJ; FERNANDA SIMAS-TOSIN, UFPR; LAURO M. SOUZA, UFPR; GUILHERME L. SASSAKI, UFPR; MAITE F. S. VASLIN, UFRJ; ELIANA BARRETO-BERGTER, UFRJ. |
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Título: |
A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 126, p. 206-216, May 2018. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2018.02.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively. MenosCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), a... Mostrar Tudo |
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Palavras-Chave: |
Peptidogalactomannan. |
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Thesagro: |
Defesa Vegetal; Glicoproteína. |
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Thesaurus Nal: |
Cladosporium herbarum. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 03116naa a2200289 a 4500
001 2103593
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2018.02.023$2DOI
100 1 $aMATTOS, B. B.
245 $aA peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells.$h[electronic resource]
260 $c2018
520 $aCladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 -> 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 -> 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae. Tobacco BY-2 cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2 cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400 mu g ml(-1) pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2 cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-l alpha, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense related gene expression at earlier times than native pGM. These results show that the side chains of the (1 -> 5) linked beta-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively.
650 $aCladosporium herbarum
650 $aDefesa Vegetal
650 $aGlicoproteína
653 $aPeptidogalactomannan
700 1 $aMONTEBIANCO, C.
700 1 $aROMANEL, E.
700 1 $aSILVA, T. da F.
700 1 $aBERNABÉ, R. B.
700 1 $aSIMAS-TOSIN, F.
700 1 $aSOUZA, L. M.
700 1 $aSASSAKI, G. L.
700 1 $aVASLIN, M. F. S.
700 1 $aBARRETO-BERGTER, E.
773 $tPlant Physiology and Biochemistry$gv. 126, p. 206-216, May 2018.
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Registro original: |
Embrapa Solos (CNPS) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
29/11/1993 |
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Data da última atualização: |
08/02/2023 |
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Autoria: |
FIGUEIREDO, F. J. C.; POPINIGIS, F. |
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Afiliação: |
FRANCISCO JOSE CAMARA FIGUEIREDO, CPATU; FLAVIO POPINIGS, AI-SEDE. |
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Título: |
Temperatura de germinação para sementes de malva (Urena lobata L.). |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1978. |
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Páginas: |
20 p. |
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Série: |
(EMBRAPA-CPATU. Comunicado técnico, 14). |
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Idioma: |
Português |
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Conteúdo: |
Para sementes da malva (Urena lobata L.), assim como para outras espécies cultivadas, as regras para análise de sementes não fazem referências quanto às condições ideais para realização do teste de germinação. Nesses casos, esse é realizado sob bases empíricas, onde procura-se dar a cada espécie condições sementlhatnes às de campo jonde é normalmente semeada. As sementes foram submetidas as diversas temperaturas de germinação, em presença e ausência de l uz. Constituiram-se tratamentos as temperaturas de gerrminação, em presença e ausência de luz. Constituiram-se tratamentos as temperaturas constantes de 20o. - 25o, 30o e 35oC e as alternadas, 20o - 30oc e 20o - 305oC. A melhor temperatura para germinação de sementes de malva, 30oC constantes, tanto na presença como na ausência da luz, apresentou maior percentagem de germinação, o mais baixo percentual de anormalidade e maior velocidade de crescimentoj de suas plântulas. |
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Palavras-Chave: |
Mallow; Seed; Test; Teste. |
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Thesagro: |
Germinação; Malva; Semente; Temperatura; Urena Lobata. |
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Thesaurus NAL: |
germination; temperature. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/57748/1/CPATU-ComTec14.pdf
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Marc: |
LEADER 01662nam a2200277 a 4500
001 1376650
005 2023-02-08
008 1978 bl uuuu u0uu1 u #d
100 1 $aFIGUEIREDO, F. J. C.
245 $aTemperatura de germinação para sementes de malva (Urena lobata L.).
260 $aBelém, PA: EMBRAPA-CPATU$c1978
300 $a20 p.
490 $a(EMBRAPA-CPATU. Comunicado técnico, 14).
520 $aPara sementes da malva (Urena lobata L.), assim como para outras espécies cultivadas, as regras para análise de sementes não fazem referências quanto às condições ideais para realização do teste de germinação. Nesses casos, esse é realizado sob bases empíricas, onde procura-se dar a cada espécie condições sementlhatnes às de campo jonde é normalmente semeada. As sementes foram submetidas as diversas temperaturas de germinação, em presença e ausência de l uz. Constituiram-se tratamentos as temperaturas de gerrminação, em presença e ausência de luz. Constituiram-se tratamentos as temperaturas constantes de 20o. - 25o, 30o e 35oC e as alternadas, 20o - 30oc e 20o - 305oC. A melhor temperatura para germinação de sementes de malva, 30oC constantes, tanto na presença como na ausência da luz, apresentou maior percentagem de germinação, o mais baixo percentual de anormalidade e maior velocidade de crescimentoj de suas plântulas.
650 $agermination
650 $atemperature
650 $aGerminação
650 $aMalva
650 $aSemente
650 $aTemperatura
650 $aUrena Lobata
653 $aMallow
653 $aSeed
653 $aTest
653 $aTeste
700 1 $aPOPINIGIS, F.
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