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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
09/12/2019 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINEZ, A. F. C.; MELLO, F. M. P.; ZUCCHI, T. D.; MELO, I. S. de; MORAES, L. A. B. |
Afiliação: |
ANA FLÁVIA CANOVAS MARTINEZ, FFCLRP-USP; FLÁVIA MADOLESI PEREIRA MELLO; TIAGO DOMINGUES ZUCCHI; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP. |
Título: |
Tandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Natural Product Research, v. 34, n. 2, p. 210-216, 2019. |
DOI: |
https://doi.org/10.1080/14786419.2018.1525713 |
Idioma: |
Inglês |
Notas: |
Arquivo com texto completo contém a versão publicada online, sem dados de volume e paginação. |
Conteúdo: |
Abstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da. |
Palavras-Chave: |
Dereplication; Luminacins; Neutral loss experiments. |
Thesagro: |
Análise Química; Bactéria; Identificação; Método de Análise. |
Thesaurus Nal: |
phytotoxins; Species identification; Streptomyces; Tandem mass spectrometry. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02000naa a2200325 a 4500 001 2116318 005 2022-06-10 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1080/14786419.2018.1525713$2DOI 100 1 $aMARTINEZ, A. F. C. 245 $aTandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL.$h[electronic resource] 260 $c2019 500 $aArquivo com texto completo contém a versão publicada online, sem dados de volume e paginação. 520 $aAbstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da. 650 $aphytotoxins 650 $aSpecies identification 650 $aStreptomyces 650 $aTandem mass spectrometry 650 $aAnálise Química 650 $aBactéria 650 $aIdentificação 650 $aMétodo de Análise 653 $aDereplication 653 $aLuminacins 653 $aNeutral loss experiments 700 1 $aMELLO, F. M. P. 700 1 $aZUCCHI, T. D. 700 1 $aMELO, I. S. de 700 1 $aMORAES, L. A. B. 773 $tNatural Product Research$gv. 34, n. 2, p. 210-216, 2019.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
20/10/2009 |
Data da última atualização: |
29/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GRISOLIA, A. B.; D´ANGELO, G. T.; PORTO NETO, L. R.; SIQUEIRA, F.; GARCIA, J. F. |
Afiliação: |
A. B. GRISOLIA, UNIVERSIDADE ESTADUAL PAULISTA/ UFGD; G. T. D´ANGELO, UNIVERSIDADE ESTADUAL PAULISTA; L. R. PORTO NETO, UNIVERSIDADE ESTADUAL PAULISTA; FABIANE SIQUEIRA, CNPGC; J. F. GARCIA, UNIVERSIDADE ESTADUAL PAULISTA. |
Título: |
Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 8, n. 3, p. 822-830, 2009. |
Idioma: |
Inglês |
Conteúdo: |
The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond?Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30 adult Nellore animals was collected; DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively. We found previously reported polymorphisms, as well as several new ones; for instance, 37 polymorphisms were found in the untranslated region segment, and in introns 1 and 2 there were one and three polymorphisms, respectively. The high degree of allelic heterogeneity in the myostatin gene could be related to its high mutation rate; it also could be the result of a long history of artificial selection for meat production, which has probably favored such modifications and maintained them in cattle populations. These polymorphisms identified in Nellore cattle could be useful for breeding programs. |
Palavras-Chave: |
Bovino de Corte; DNA sequencing; Nelore. |
Thesagro: |
Carne; DNA; Melhoramento genético animal. |
Thesaurus NAL: |
Animal breeding; Double muscling; Nucleotides. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171774/1/Myostatin-GDF8-single-nucleotide.pdf
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Marc: |
LEADER 02284naa a2200277 a 4500 001 1572633 005 2018-01-29 008 2009 bl uuuu u00u1 u #d 100 1 $aGRISOLIA, A. B. 245 $aMyostatin (GDF8) single nucleotide polymorphisms in Nellore cattle.$h[electronic resource] 260 $c2009 520 $aThe myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond?Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30 adult Nellore animals was collected; DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively. We found previously reported polymorphisms, as well as several new ones; for instance, 37 polymorphisms were found in the untranslated region segment, and in introns 1 and 2 there were one and three polymorphisms, respectively. The high degree of allelic heterogeneity in the myostatin gene could be related to its high mutation rate; it also could be the result of a long history of artificial selection for meat production, which has probably favored such modifications and maintained them in cattle populations. These polymorphisms identified in Nellore cattle could be useful for breeding programs. 650 $aAnimal breeding 650 $aDouble muscling 650 $aNucleotides 650 $aCarne 650 $aDNA 650 $aMelhoramento genético animal 653 $aBovino de Corte 653 $aDNA sequencing 653 $aNelore 700 1 $aD´ANGELO, G. T. 700 1 $aPORTO NETO, L. R. 700 1 $aSIQUEIRA, F. 700 1 $aGARCIA, J. F. 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 8, n. 3, p. 822-830, 2009.
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