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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/04/2015 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
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Título: |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 6, e98758, 2014. |
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DOI: |
https://doi.org/10.1371/journal.pone.0098758 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Palavras-Chave: |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122336/1/Cnpgl-2014-PlosOne-Evaluation.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1371/journal.pone.0098758$2DOI
100 1 $aDORNELES, E. M. S.
245 $aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
653 $aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
700 1 $aSANTANA, J. A.
700 1 $aRIBEIRO, D.
700 1 $aDORELLA, F. A.
700 1 $aGUIMARAES, A. S.
700 1 $aMOAWAD, M. S.
700 1 $aSELIM, S. A.
700 1 $aGARALDI, A. L. M.
700 1 $aMIYOSHI, A.
700 1 $aRIBEIRO, M. G.
700 1 $aGOUVEIA, A. M. G.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tPlos One$gv. 9, n. 6, e98758, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 65 | |
| 61. |  | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 62. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N. de; LAGE, A. P.; BLAGITZ, M. G.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 63. | | DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. Plos One, v. 9, n. 6, e98758, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 64. | | DINIZ NETO, H. C.; LOMBARDI, N. C.; CAMPOS, M. M.; LAGE, A. P.; SILVA, R. O. S.; DORNELES, E. M. S.; LAGE, C. F. A.; CARVALHO, W. A.; MACHADO, F. S.; PEREIRA, L. G. R.; TOMICH, T. R.; RAMOS, C. P.; ASSIS, R. A.; LOBATO, F. C. F.; SANTANA, J. A.; SANTOS, E. L. S.; ANDRADE, R. S.; COELHO, S. G. Effects of vaccination against brucellosis and clostridia on the intake, performance, feeding behavior, blood parameters, and immune responses of dairy heifers calves. Journal of Animal Science, v. 99, n. 5, skab107, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 65. | | DORNELES, E. M. S.; GUIMARÃES, A. S.; GOUVEIA, A. M. G.; COURA, F. M.; CARMO, F. B. do; PAULETTI, R. B.; AZEVEDO, V.; LILENBAUM, W.; VITOR, V. de A.; PINHEIRO, R. R.; FERREIRA, A. C. R.; DASSO, M. G.; LAGE, A. P.; HEINEMANN, M. B. Seroprevalence of Brucella ovis-epididymitis, smooth-Brucella, leptospirosis, toxoplasmosis, and Maedi-Visna in sheep slaughtered in Minas Gerais State, Brazil. Brazilian Journal of Veterinary Research and Animal Science, v. 57, n. 2, e164278, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 65 | |
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