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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
27/06/2023 |
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Autoria: |
JANSON, P. O.; AMATO, F.; WEISS, T. J.; RALPH, M. H.; SEAMARK, R. F. |
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Título: |
On the isolated perfused sheep ovary as a model for the study of ovarian function. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
American Fertility Society, v. 30, n. 2, p. 230-236, Aug. 978. |
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DOI: |
https://doi.org/10.1016/S0015-0282(16)43465-5 |
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Idioma: |
Inglês |
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Conteúdo: |
A technique for in vitro perfusion of sheep ovaries is described and the effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. Perfusion with medium 199, hormone-free and made isotonic with plasma, caused swelling and rupture of follicles in 3 hours regardless of the stage of the cycle. The ruptures were accompanied by a stromal edema. The swelling and rupture of the follicles as well as the edema of the stroma were effectively prevented by increasing the colloid osmotic pressure of the medium by adding dextran and dextrose. By contrast, perfusion with plasma curtailed follicular swelling but not stromal edema. The results indicate that follicular ruptures observed in perfusion systems may be artifacts and that careful selection of the perfusion medium is needed to avoid misinterpretation. Ovarian endocrine function as assessed by determination of progesterone, testosterone, and estrogen was sustained at levels similar to those found in situ in ovaries perfused with oophorectomized ewe plasma, but was considerably reduced with either medium 199 or medium 199 plus dextran and dextrose as the perfusion media. |
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Palavras-Chave: |
Hydrogen-Ion Concentration; Ovary. |
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Thesagro: |
Fisiologia; Ovino; Reprodução. |
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Thesaurus Nal: |
Glucose; Lactates; Metabolism; Muscle contraction; Oxygen; Physiology; Reproduction; Sheep; Testosterone. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02174naa a2200349 a 4500
001 1522298
005 2023-06-27
008 1978 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/S0015-0282(16)43465-5$2DOI
100 1 $aJANSON, P. O.
245 $aOn the isolated perfused sheep ovary as a model for the study of ovarian function.$h[electronic resource]
260 $c1978
520 $aA technique for in vitro perfusion of sheep ovaries is described and the effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. Perfusion with medium 199, hormone-free and made isotonic with plasma, caused swelling and rupture of follicles in 3 hours regardless of the stage of the cycle. The ruptures were accompanied by a stromal edema. The swelling and rupture of the follicles as well as the edema of the stroma were effectively prevented by increasing the colloid osmotic pressure of the medium by adding dextran and dextrose. By contrast, perfusion with plasma curtailed follicular swelling but not stromal edema. The results indicate that follicular ruptures observed in perfusion systems may be artifacts and that careful selection of the perfusion medium is needed to avoid misinterpretation. Ovarian endocrine function as assessed by determination of progesterone, testosterone, and estrogen was sustained at levels similar to those found in situ in ovaries perfused with oophorectomized ewe plasma, but was considerably reduced with either medium 199 or medium 199 plus dextran and dextrose as the perfusion media.
650 $aGlucose
650 $aLactates
650 $aMetabolism
650 $aMuscle contraction
650 $aOxygen
650 $aPhysiology
650 $aReproduction
650 $aSheep
650 $aTestosterone
650 $aFisiologia
650 $aOvino
650 $aReprodução
653 $aHydrogen-Ion Concentration
653 $aOvary
700 1 $aAMATO, F.
700 1 $aWEISS, T. J.
700 1 $aRALPH, M. H.
700 1 $aSEAMARK, R. F.
773 $tAmerican Fertility Society$gv. 30, n. 2, p. 230-236, Aug. 978.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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