02174naa a2200349 a 450000100080000000500110000800800410001902400550006010000180011524501100013326000090024352012220025265000120147465000130148665000150149965000230151465000110153765000150154865000170156365000100158065000170159065000150160765000100162265000170163265300310164965300100168070000140169070000170170470000170172170000190173877300670175715222982023-06-27       1978    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/S0015-0282(16)43465-52DOI1 aJANSON, P. O.  aOn the isolated perfused sheep ovary as a model for the study of ovarian function.h[electronic resource]  c1978  aA technique for in vitro perfusion of sheep ovaries is described and the effect of perfusion with different media on ovarian function and, in particular, on the phenomenon of follicular rupture and swelling is examined. Perfusion with medium 199, hormone-free and made isotonic with plasma, caused swelling and rupture of follicles in 3 hours regardless of the stage of the cycle. The ruptures were accompanied by a stromal edema. The swelling and rupture of the follicles as well as the edema of the stroma were effectively prevented by increasing the colloid osmotic pressure of the medium by adding dextran and dextrose. By contrast, perfusion with plasma curtailed follicular swelling but not stromal edema. The results indicate that follicular ruptures observed in perfusion systems may be artifacts and that careful selection of the perfusion medium is needed to avoid misinterpretation. Ovarian endocrine function as assessed by determination of progesterone, testosterone, and estrogen was sustained at levels similar to those found in situ in ovaries perfused with oophorectomized ewe plasma, but was considerably reduced with either medium 199 or medium 199 plus dextran and dextrose as the perfusion media.  aGlucose  aLactates  aMetabolism  aMuscle contraction  aOxygen  aPhysiology  aReproduction  aSheep  aTestosterone  aFisiologia  aOvino  aReprodução  aHydrogen-Ion Concentration  aOvary1 aAMATO, F.1 aWEISS, T. J.1 aRALPH, M. H.1 aSEAMARK, R. F.  tAmerican Fertility Societygv. 30, n. 2, p. 230-236, Aug. 978.