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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Suínos e Aves. |
Data corrente: |
09/09/2021 |
Data da última atualização: |
15/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HUL, L. M.; IBELLI, A. M. G.; SAVOLDI, I. R.; MARCELINO, D. E. P.; FERNANDES, L. T.; PEIXOTO, J. O.; CANTAO, M. E.; HIGA, R. H.; GIACHETTO, P. F.; COUTINHO, L. L.; LEDUR, M. C. |
Afiliação: |
LUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA. |
Título: |
Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientific Reports, v. 11, n. 1, p. 1-13. 2021. |
DOI: |
https://doi.org/10.1038/s41598-021-97306-3 |
Idioma: |
Inglês |
Notas: |
Article number: 17965. |
Conteúdo: |
Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans. |
Palavras-Chave: |
Análises de expressão diferencial; Cartilagem da cabeça do fêmur; Differential expression analysis; Expressão gênica; Reação da cadeia de polimerase; Separação da cabeça femoral; Sequenciamento de RNA; Transcriptoma da cartilagem do fêmur. |
Thesagro: |
Frango; Frango de Corte. |
Thesaurus Nal: |
Broiler chickens; Gene expression; Quantitative polymerase chain reaction; Transcriptome. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226988/1/final9712.pdf
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Marc: |
LEADER 02920naa a2200433 a 4500 001 2135377 005 2021-10-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-021-97306-3$2DOI 100 1 $aHUL, L. M. 245 $aDifferentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.$h[electronic resource] 260 $c2021 500 $aArticle number: 17965. 520 $aLocomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans. 650 $aBroiler chickens 650 $aGene expression 650 $aQuantitative polymerase chain reaction 650 $aTranscriptome 650 $aFrango 650 $aFrango de Corte 653 $aAnálises de expressão diferencial 653 $aCartilagem da cabeça do fêmur 653 $aDifferential expression analysis 653 $aExpressão gênica 653 $aReação da cadeia de polimerase 653 $aSeparação da cabeça femoral 653 $aSequenciamento de RNA 653 $aTranscriptoma da cartilagem do fêmur 700 1 $aIBELLI, A. M. G. 700 1 $aSAVOLDI, I. R. 700 1 $aMARCELINO, D. E. P. 700 1 $aFERNANDES, L. T. 700 1 $aPEIXOTO, J. O. 700 1 $aCANTAO, M. E. 700 1 $aHIGA, R. H. 700 1 $aGIACHETTO, P. F. 700 1 $aCOUTINHO, L. L. 700 1 $aLEDUR, M. C. 773 $tScientific Reports$gv. 11, n. 1, p. 1-13. 2021.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
10/01/2008 |
Data da última atualização: |
09/10/2008 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Circulação/Nível: |
-- - -- |
Autoria: |
PAGLIARINI, M. S.; VALLE, C. B. do; BATISTA, L. A. R.; BONATO, A. B. M.; BOLDRINI, K. R.; ADAMOWSKI, E. de V.; SILVA, N. da; FUZINATTO, V. A.; FELISMINO, M. F.; PASCOTTO, C. R.; MESSAGE, H. J.; CALISTO, V. |
Afiliação: |
Maria Suely Pagliarini, UEM; Cacilda Borges do Valle, CNPGC; Luiz Aberto Rocha Batista, CPPSE; Andréa Beatriz Mendes Bonato, UEM; Kellen Regina Boldrini, UEM; Eleniza de Victor Adamowski, UEM; Neide da Silva, UEM; Veridiana Aparecida Fuzinatto, UEM; Mariana Ferrari Felismino, UEM; Claudicéia Risso Pascotto, UEM; Hugo José Message, UEM; Vergílio Calisto, UEM. |
Título: |
Cytogenetics of Brachiaria and Paspalum in relation to breeding. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2007, Campo Grande, MS. Palestras_resumos [do]... Campo Grande, MS: Embrapa Gado de Corte, 2007. |
Páginas: |
24 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Inglês |
Palavras-Chave: |
Feed Crops; Feed grasses. |
Thesagro: |
Brachiaria; Citogenética Vegetal; Gramínea Forrageira; Melhoramento Genético Vegetal; Planta Forrageira. |
Thesaurus NAL: |
cytogenetics; Paspalum; plant breeding. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01144naa a2200373 a 4500 001 1325926 005 2008-10-09 008 2007 bl uuuu u00u1 u #d 100 1 $aPAGLIARINI, M. S. 245 $aCytogenetics of Brachiaria and Paspalum in relation to breeding. 260 $c2007 300 $a24 p.$c1 CD-ROM. 650 $acytogenetics 650 $aPaspalum 650 $aplant breeding 650 $aBrachiaria 650 $aCitogenética Vegetal 650 $aGramínea Forrageira 650 $aMelhoramento Genético Vegetal 650 $aPlanta Forrageira 653 $aFeed Crops 653 $aFeed grasses 700 1 $aVALLE, C. B. do 700 1 $aBATISTA, L. A. R. 700 1 $aBONATO, A. B. M. 700 1 $aBOLDRINI, K. R. 700 1 $aADAMOWSKI, E. de V. 700 1 $aSILVA, N. da 700 1 $aFUZINATTO, V. A. 700 1 $aFELISMINO, M. F. 700 1 $aPASCOTTO, C. R. 700 1 $aMESSAGE, H. J. 700 1 $aCALISTO, V. 773 $tIn: SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2007, Campo Grande, MS. Palestras_resumos [do]... Campo Grande, MS: Embrapa Gado de Corte, 2007.
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