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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/04/2015 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
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Título: |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 6, e98758, 2014. |
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DOI: |
https://doi.org/10.1371/journal.pone.0098758 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Palavras-Chave: |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122336/1/Cnpgl-2014-PlosOne-Evaluation.pdf
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Marc: |
LEADER 02105naa a2200301 a 4500
001 2013504
005 2024-01-30
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0098758$2DOI
100 1 $aDORNELES, E. M. S.
245 $aEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.$h[electronic resource]
260 $c2014
520 $aThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
653 $aEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
700 1 $aSANTANA, J. A.
700 1 $aRIBEIRO, D.
700 1 $aDORELLA, F. A.
700 1 $aGUIMARAES, A. S.
700 1 $aMOAWAD, M. S.
700 1 $aSELIM, S. A.
700 1 $aGARALDI, A. L. M.
700 1 $aMIYOSHI, A.
700 1 $aRIBEIRO, M. G.
700 1 $aGOUVEIA, A. M. G.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tPlos One$gv. 9, n. 6, e98758, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/08/2005 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, A. V.; SOUZA SOBRINHO, F. de; VALLE, C. B. do; LEDO, F. J. da S.; BOTREL, M. de A.; OLIVEIRA, J. da S. e; XAVIER, D. F. |
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Afiliação: |
ANTONIO VANDER PEREIRA, CNPGL; FAUSTO DE SOUZA SOBRINHO, CNPGL; CACILDA BORGES DO VALLE; FRANCISCO JOSE DA SILVA LEDO, CNPGL; MILTON DE ANDRADE BOTREL; JACKSON SILVA E OLIVEIRA; DEISE FERREIRA XAVIER, CNPGL. |
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Título: |
Selection of interspecific Brachiaria hybrids to intensify milk production on pastures. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 5, n. 1, p. 99-104, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Milk production in Brazil is largely based on pasture utilization which represents an in expensive feed compared to processed or conserved forages. Brachiaria brizantha and B. decumbens are prevalent in Brazil owing to their high yield potential and good adaptation to acid and infertile soils. Their forage quality is, however, considered low in view of the nutritional needs of animals of high genetic potential for milk production. Fortunately, there is intra and interspecific variation for forage quality in Brachiaria germplasm to be exploited in the development of new cultivars. This paper reports on the evaluation of 47 interspecific hybrids between B. ruziziensis (R) × B. brizantha (B) and R × B. decumbens (D), with the objective of selecting potential new cultivars that combine high yield and forage quality. The experiment was arranged in a completely randomized design with two replicates and two common cultivars as controls. Data of the total dry matter yield, dry matter percentage, plant height and regrowth vigour of six cuts were collected. Samples from the third cut were analysed for nutritive value using near infrared spectroscopy. Results confirmed the existence of variability for the majority of the traits analysed so high quality and productive hybrids can be selected. |
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Palavras-Chave: |
B brizantha; B ruziziensis; brizantha; Melhoramento genético; Pasto; Qualidade de forragem; ruziziensis. |
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Thesagro: |
Brachiaria Decumbens; Forragem; Pastagem; Qualidade; Semente. |
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Thesaurus NAL: |
breeding; forage quality; pastures. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/594880/1/Selection-of-interspecific-Brachiaria-hybrids-to-intensify-milk-production-on-pastures.pdf
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Marc: |
LEADER 02317naa a2200373 a 4500
001 1594880
005 2022-08-09
008 2005 bl uuuu u00u1 u #d
100 1 $aPEREIRA, A. V.
245 $aSelection of interspecific Brachiaria hybrids to intensify milk production on pastures.
260 $c2005
520 $aMilk production in Brazil is largely based on pasture utilization which represents an in expensive feed compared to processed or conserved forages. Brachiaria brizantha and B. decumbens are prevalent in Brazil owing to their high yield potential and good adaptation to acid and infertile soils. Their forage quality is, however, considered low in view of the nutritional needs of animals of high genetic potential for milk production. Fortunately, there is intra and interspecific variation for forage quality in Brachiaria germplasm to be exploited in the development of new cultivars. This paper reports on the evaluation of 47 interspecific hybrids between B. ruziziensis (R) × B. brizantha (B) and R × B. decumbens (D), with the objective of selecting potential new cultivars that combine high yield and forage quality. The experiment was arranged in a completely randomized design with two replicates and two common cultivars as controls. Data of the total dry matter yield, dry matter percentage, plant height and regrowth vigour of six cuts were collected. Samples from the third cut were analysed for nutritive value using near infrared spectroscopy. Results confirmed the existence of variability for the majority of the traits analysed so high quality and productive hybrids can be selected.
650 $abreeding
650 $aforage quality
650 $apastures
650 $aBrachiaria Decumbens
650 $aForragem
650 $aPastagem
650 $aQualidade
650 $aSemente
653 $aB brizantha
653 $aB ruziziensis
653 $abrizantha
653 $aMelhoramento genético
653 $aPasto
653 $aQualidade de forragem
653 $aruziziensis
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aVALLE, C. B. do
700 1 $aLEDO, F. J. da S.
700 1 $aBOTREL, M. de A.
700 1 $aOLIVEIRA, J. da S. e
700 1 $aXAVIER, D. F.
773 $tCrop Breeding and Applied Biotechnology$gv. 5, n. 1, p. 99-104, 2005.
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