Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/01/2002 |
Data da última atualização: |
10/05/2023 |
Autoria: |
DOMINGUEZ, A.; GUERRI, J.; CAMBRA, M.; NAVARRO, L.; MORENO, P.; PENA, L. |
Título: |
Efficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Plant Cell, v.19, n.4, p.427-433, 2000. |
Idioma: |
Inglês |
Conteúdo: |
The coat protein gene of citrus tristeza virus (CTV) has been introduced into Mexican lime (Citrus aurantifolia Swing.) plants by using an improved Agrobacterium-mediated genetic trasformation system. Internodal stem segments from greenhouse-grown seedlings were co-cultivated with A. tumefaciens strain EHA-105 carrying the binary plasmid pBI 121/CTV-CP in a medium rich in auxins that provided the explant cells with the proper tratament to shift them to a competent state for transformation. |
Palavras-Chave: |
coat protein; transformation. |
Thesaurus Nal: |
Agrobacterium; Citrus; Citrus tristeza virus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01172naa a2200241 a 4500 001 1650353 005 2023-05-10 008 2000 bl uuuu u00u1 u #d 100 1 $aDOMINGUEZ, A. 245 $aEfficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus.$h[electronic resource] 260 $c2000 520 $aThe coat protein gene of citrus tristeza virus (CTV) has been introduced into Mexican lime (Citrus aurantifolia Swing.) plants by using an improved Agrobacterium-mediated genetic trasformation system. Internodal stem segments from greenhouse-grown seedlings were co-cultivated with A. tumefaciens strain EHA-105 carrying the binary plasmid pBI 121/CTV-CP in a medium rich in auxins that provided the explant cells with the proper tratament to shift them to a competent state for transformation. 650 $aAgrobacterium 650 $aCitrus 650 $aCitrus tristeza virus 653 $acoat protein 653 $atransformation 700 1 $aGUERRI, J. 700 1 $aCAMBRA, M. 700 1 $aNAVARRO, L. 700 1 $aMORENO, P. 700 1 $aPENA, L. 773 $tPlant Cell$gv.19, n.4, p.427-433, 2000.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |