01172naa a2200241 a 450000100080000000500110000800800410001910000180006024501350007826000090021352004990022265000180072165000110073965000260075065300170077665300190079370000150081270000150082770000160084270000150085870000130087377300440088616503532023-05-10       2000    bl uuuu     u00u1 u    #d1 aDOMINGUEZ, A.  aEfficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus.h[electronic resource]  c2000  aThe coat protein gene of citrus tristeza virus (CTV) has been introduced into Mexican lime (Citrus aurantifolia Swing.) plants by using an improved Agrobacterium-mediated genetic trasformation system. Internodal stem segments from greenhouse-grown seedlings were co-cultivated with A. tumefaciens strain EHA-105 carrying the binary plasmid pBI 121/CTV-CP in a medium rich in auxins that provided the explant cells with the proper tratament to shift them to a competent state for transformation.  aAgrobacterium  aCitrus  aCitrus tristeza virus  acoat protein  atransformation1 aGUERRI, J.1 aCAMBRA, M.1 aNAVARRO, L.1 aMORENO, P.1 aPENA, L.  tPlant Cellgv.19, n.4, p.427-433, 2000.