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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
13/11/2020 |
Data da última atualização: |
18/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GODOY, C. V.; MEYER, M. C.; LOPES, I. de O. N.; UTIAMADA, C. V.; CAMPOS, H. D.; DIAS, A. R.; NUNES JUNIOR, J.; PIMENTA, C. B.; SICHOCKI, D.; LIMA, L. A. de S.; MOREIRA, E. N.; KONAGESKI, T. E.; KONAGESKI, F. T.; GRIGOLLI, J. F. J.; BELUFI, L. M. de R.; CARREGAL, L. H.; GOUSSAN JÚNIOR M.; DIAS, M. D.; MARTINS, M. C.; MÜLLER, M. A.; ARRUDA, J. H.; CARLIN, V. J. |
Afiliação: |
CLAUDIA VIEIRA GODOY, CNPSO; MAURICIO CONRADO MEYER, CNPSO; IVANI DE OLIVEIRA NEGRAO LOPES, CNPSO; Carlos Vlitinori Utiamada, Tagro Tecnologia Agropecuária; Hercules Diniz Campos, UniRV/ Campos Pesquisa Agrícola; Alfredo R. Dias, Fundaçäo Chapadão; José Nunes Junior, CTPA/ Emater - G0; Cláudia Barbosa Pimenta, CTPA/ Emater - G0; Diego Sichocki, Meta Consultoria Agrícola; Luís Antônio de Sousa Lima, Meta Consultoria Agrícola; Eder Novaes Moreira, Fitolab Pesquisa e Desenvolvimento Agrícola; Tiago E. Konageski, Rural Técnica Experimentos Agronômicos Ltda.; Fabíola T. Konageski, Rural Técnica Experimentos Agronômicos Ltda; José Fernando J. Grigolli, Fundação MS; Luana M. de R. Belufi, Fundaçäo Rio Verde; Luís Henrique Carregal, Agro Carregal Pesquisa e Proteção de Plantas Eireli; Marcio Goussain Júnior, Assist Consultoria; Moab Diany Dias, Universidade Federal do Tocantins; Mônica Cagnin Martins, Círculo Verde Assessoria Agronômica e Pesquisa; Mônica A, Müller, FUNDAÇÃO MT; JOSICLEA H. ARRUDA, FUNDAÇÃO MT; Valtemir J. Carlin, Agrodinâmica Pesquisa e Consultoria Agropecuária. |
Título: |
Fungicidas ao alvo. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Cultivar grandes culturas, v. 20 , n. 253, p. 18-22, jun. 2020. |
Idioma: |
Português |
Palavras-Chave: |
Manchas-alvo. |
Thesagro: |
Fungicida; Fungo Para Controle Biológico; Produtividade; Soja. |
Thesaurus Nal: |
Fungicides; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01182naa a2200445 a 4500 001 2126582 005 2020-12-18 008 2020 bl uuuu u00u1 u #d 100 1 $aGODOY, C. V. 245 $aFungicidas ao alvo.$h[electronic resource] 260 $c2020 650 $aFungicides 650 $aSoybeans 650 $aFungicida 650 $aFungo Para Controle Biológico 650 $aProdutividade 650 $aSoja 653 $aManchas-alvo 700 1 $aMEYER, M. C. 700 1 $aLOPES, I. de O. N. 700 1 $aUTIAMADA, C. V. 700 1 $aCAMPOS, H. D. 700 1 $aDIAS, A. R. 700 1 $aNUNES JUNIOR, J. 700 1 $aPIMENTA, C. B. 700 1 $aSICHOCKI, D. 700 1 $aLIMA, L. A. de S. 700 1 $aMOREIRA, E. N. 700 1 $aKONAGESKI, T. E. 700 1 $aKONAGESKI, F. T. 700 1 $aGRIGOLLI, J. F. J. 700 1 $aBELUFI, L. M. de R. 700 1 $aCARREGAL, L. H. 700 1 $aGOUSSAN JÚNIOR M. 700 1 $aDIAS, M. D. 700 1 $aMARTINS, M. C. 700 1 $aMÜLLER, M. A. 700 1 $aARRUDA, J. H. 700 1 $aCARLIN, V. J. 773 $tCultivar grandes culturas$gv. 20 , n. 253, p. 18-22, jun. 2020.
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
24/04/2021 |
Data da última atualização: |
12/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
ISSN: |
1874-3919 |
DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
Idioma: |
Inglês Português |
Notas: |
Na publicação: Angela Mehta. |
Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
Thesaurus NAL: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 03484naa a2200469 a 4500 001 2131497 005 2021-07-12 008 2021 bl uuuu u00u1 u #d 022 $a1874-3919 024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI 100 1 $aTÁVORA, F. T. P. K. 245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource] 260 $c2021 500 $aNa publicação: Angela Mehta. 520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. 650 $ablast disease 650 $adisease resistance 650 $afoliar diseases 650 $aMagnaporthe oryzae 650 $aplant diseases and disorders 650 $aproteomics 650 $arice 650 $aArroz 650 $aBrusone 650 $aDoença de Planta 650 $aOryza Sativa 653 $aGene ontology 653 $aPlant immunity 653 $aPTO-based TIGS 653 $aRT-qPCR 653 $aS-gene 653 $aTransient-Induced gene silencing 700 1 $aBEVITORI, R. 700 1 $aMELLO, R. N. de 700 1 $aCINTRA, M. M. D. F. 700 1 $aOLIVEIRA NETO, O. B. 700 1 $aFONTES, W. 700 1 $aCASTRO, M. S. 700 1 $aSOUSA, M. V. 700 1 $aFRANCO, O. L. 700 1 $aREIS, A. M. dos 773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
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