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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
30/11/2023 |
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Autoria: |
GIMENEZ, D. F. |
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Título: |
Staining rickettsiae in yolk sac culture. |
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Ano de publicação: |
1964 |
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Fonte/Imprenta: |
Stain Technology, v. 39, n. 3, p. 135-140, 1964. |
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DOI: |
https://doi.org/10.3109/10520296409061219 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rickettsiae in yolk sacs are not stained well by the Macchiavello technique, and experiments were undertaken to understand the mechanisms involved. It was found that the citric acid destaining step was not effective and that most of the basic fuchsin was lost from the rickettsiae during the application of methylene blue, another basic dye. A staining technique was then evolved with carbol basic fuchsin in pH 7.45 phosphate buffer (0.4% dye, 0.4% phenol, 0.07 M buffer), followed directly by 0.8% aqueous malachite green oxalate. This technique worked well for R. mooseri, R. prowazeki, R. rickettsii, R. akari, and R. buretii, but for R. tsutsugumushi a modification was needed, whereby 4% aqueous Fe(NO3)3·9H2O was used as destaining solution, and 0.5% aqueous fast green as the counter-stain. |
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Palavras-Chave: |
Coloring Agents; Staining and Labeling; Tissue Culture Techniques. |
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Thesagro: |
Animal; Bacteriologia; Doença. |
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Thesaurus Nal: |
Animal diseases; Culture media; Rickettsia; Yolk sac. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01489naa a2200253 a 4500
001 1523248
005 2023-11-30
008 1964 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3109/10520296409061219$2DOI
100 1 $aGIMENEZ, D. F.
245 $aStaining rickettsiae in yolk sac culture.
260 $c1964
520 $aAbstract: Rickettsiae in yolk sacs are not stained well by the Macchiavello technique, and experiments were undertaken to understand the mechanisms involved. It was found that the citric acid destaining step was not effective and that most of the basic fuchsin was lost from the rickettsiae during the application of methylene blue, another basic dye. A staining technique was then evolved with carbol basic fuchsin in pH 7.45 phosphate buffer (0.4% dye, 0.4% phenol, 0.07 M buffer), followed directly by 0.8% aqueous malachite green oxalate. This technique worked well for R. mooseri, R. prowazeki, R. rickettsii, R. akari, and R. buretii, but for R. tsutsugumushi a modification was needed, whereby 4% aqueous Fe(NO3)3·9H2O was used as destaining solution, and 0.5% aqueous fast green as the counter-stain.
650 $aAnimal diseases
650 $aCulture media
650 $aRickettsia
650 $aYolk sac
650 $aAnimal
650 $aBacteriologia
650 $aDoença
653 $aColoring Agents
653 $aStaining and Labeling
653 $aTissue Culture Techniques
773 $tStain Technology$gv. 39, n. 3, p. 135-140, 1964.
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Embrapa Caprinos e Ovinos (CNPC) |
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