01512naa a2200253 a 450000100080000000500110000800800410001902400510006010000190011124500690013026000090019952008140020865000200102265000180104265000150106065000130107565000110108865000180109965000120111765300200112965300260114965300300117577300530120515232482025-06-07       1964    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3109/105202964090612192DOI1 aGIMENEZ, D. F.  aStaining rickettsiae in yolk sac culture.h[electronic resource]  c1964  aAbstract: Rickettsiae in yolk sacs are not stained well by the Macchiavello technique, and experiments were undertaken to understand the mechanisms involved. It was found that the citric acid destaining step was not effective and that most of the basic fuchsin was lost from the rickettsiae during the application of methylene blue, another basic dye. A staining technique was then evolved with carbol basic fuchsin in pH 7.45 phosphate buffer (0.4% dye, 0.4% phenol, 0.07 M buffer), followed directly by 0.8% aqueous malachite green oxalate. This technique worked well for R. mooseri, R. prowazeki, R. rickettsii, R. akari, and R. buretii, but for R. tsutsugumushi a modification was needed, whereby 4% aqueous Fe(NO3)3·9H2O was used as destaining solution, and 0.5% aqueous fast green as the counter-stain.  aAnimal diseases  aCulture media  aRickettsia  aYolk sac  aAnimal  aBacteriologia  aDoença  aColoring Agents  aStaining and Labeling  aTissue Culture Techniques  tStain Technologygv. 39, n. 3, p. 135-140, 1964.