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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/11/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MONTEIRO, J. P.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; ANDRÉ, W. P. P.; RIBEIRO, W. L. C.; MELO, A. C. F. L.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. |
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Afiliação: |
JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) -Sobral, CE, Brazil; Gracielle Araújo Frota; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Ana Carolina Fonseca Lindoso Melo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil. |
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Título: |
Molecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588. |
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Idioma: |
Inglês |
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Notas: |
In conjunction with 53rd MSPTM Annual Conference. |
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Conteúdo: |
Abstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period. MenosAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a sh... Mostrar Tudo |
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Palavras-Chave: |
Beta tubulin; Dosage effects; Resistance to anthelmintics; SNP. |
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Thesagro: |
Anti-Helmíntico; Haemonchus contortus; Resistência química. |
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Thesaurus Nal: |
Anthelmintics; Drug resistance; Oxfendazole. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166452/1/CNPC-2017-Molecular.pdf
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Marc: |
LEADER 02735nam a2200349 a 4500
001 2079254
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aMONTEIRO, J. P.
245 $aMolecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588.$c4588
500 $aIn conjunction with 53rd MSPTM Annual Conference.
520 $aAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period.
650 $aAnthelmintics
650 $aDrug resistance
650 $aOxfendazole
650 $aAnti-Helmíntico
650 $aHaemonchus contortus
650 $aResistência química
653 $aBeta tubulin
653 $aDosage effects
653 $aResistance to anthelmintics
653 $aSNP
700 1 $aSANTOS, J. M. L. dos
700 1 $aVASCONCELOS, J. F.
700 1 $aFROTA, G. A.
700 1 $aANDRÉ, W. P. P.
700 1 $aRIBEIRO, W. L. C.
700 1 $aMELO, A. C. F. L.
700 1 $aTEIXEIRA, M.
700 1 $aVIEIRA, L. da S.
700 1 $aBEVILAQUA, C. M. L.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 53 | |
| 5. |  | MORAIS, O. P. de; GUIMARÃES, C. M.; CASTRO, A. P.; MORAIS JÚNIOR, O. P. de. Avaliação da população de arroz de terras altas CNA6, quanto ao seu potencial de melhoramento visando tolerância à deficiência hídrica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 7. | | GUIMARÃES, C. M.; STONE, L. F.; CASTRO, A. P. de; MORAIS JUNIOR, O. P. de. Physiological parameters to select upland rice genotypes for tolerance to water deficit. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 7, p. 534-540, jul. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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| 9. | | GUIMARÃES, C. M.; BRESEGHELLO, F.; CASTRO, A. P. de; STONE, L. F.; MORAIS JÚNIOR, O. P. de. Avaliação de arroz de terras altas do grupo indica, sob condições de irrigação adequada e de deficiência hídrica. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 6., 2009, Porto Alegre. Estresses e sustentabilidade: desafios para a lavoura arrozeira: anais. Porto Alegre: Palotti, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | MORAIS JÚNIOR, O. P. de; GUIMARÃES, P. H. R.; MORAIS, O. P. de; PEREIRA, J. A.; MELO, P. G. S. Análise dialélica parcial generalizada com linhagens de arroz vermelho e branco em dois ambientes. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | MORAIS JÚNIOR, O. P. de; GUIMARÃES, P. H. R.; MORAIS, O. P. de; PEREIRA, J. A.; MELO, P. G. S. Análise dialélica parcial generalizada com linhagens de arroz vermelho e branco em dois ambientes. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | MORAIS JÚNIOR, O. P. de; MORAIS, O. P. de; BRESEGHELLO, F.; RANGEL, P. H. N.; MAGALHÃES JUNIOR, A. M. de. Comparação de índices de seleção aplicados em seleção recorrente de arroz irrigado. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 9., 2015, Pelotas. Ciência e tecnologia para otimização da orizicultura: anais. Brasília, DF: Embrapa; Pelotas: Sosbai, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Clima Temperado. |
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| 16. | | MORAIS JÚNIOR, O. P. de; MELO, P. G. S.; MORAIS, O. P. de; COLOMBARI FILHO, J. M. Genetic variability during four cycles of recurrent selection in rice. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n, 11, p. 1033-1041, nov. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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| 19. | | BARROS, M. S. de; MORAIS JÚNIOR, O. P. de; MORAIS, O. P. de; TORGA, P. P.; MELO, P. G. S. Ganho genético simultâneo em progênies de arroz de terras altas por meio de índice de seleção. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 53 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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