02735nam a2200349 a 450000100080000000500110000800800410001910000200006024501330008026002040021350000540041752015200047165000180199165000200200965000160202965000210204565000250206665000260209165300170211765300190213465300320215365300080218570000250219370000230221870000170224170000210225870000220227970000220230170000170232370000210234070000240236120792542019-09-23       2017    bl uuuu     u00u1 u    #d1 aMONTEIRO, J. P.  aMolecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate.h[electronic resource]  aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588.c2017  aIn conjunction with 53rd MSPTM Annual Conference.  aAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period.  aAnthelmintics  aDrug resistance  aOxfendazole  aAnti-Helmíntico  aHaemonchus contortus  aResistência química  aBeta tubulin  aDosage effects  aResistance to anthelmintics  aSNP1 aSANTOS, J. M. L. dos1 aVASCONCELOS, J. F.1 aFROTA, G. A.1 aANDRÉ, W. P. P.1 aRIBEIRO, W. L. C.1 aMELO, A. C. F. L.1 aTEIXEIRA, M.1 aVIEIRA, L. da S.1 aBEVILAQUA, C. M. L.